نتایج جستجو برای: double strand break

تعداد نتایج: 297026  

Journal: :Nucleic acids research 1992
B S Lopez P Bertrand-Mercat E Corteggiani J Coppey

The effect of the structure of donor DNA molecules on the initiation of recombination for double strand break repair in human nuclear extracts, was investigated here. A unique double strand break was introduced into M13 duplex derivatives by digestion with restriction enzymes. After coincubation of the cleaved DNA in human nuclear extracts, with a plasmid containing M13 sequences spanning the b...

2010
Aymeric P. Bailly Alasdair Freeman Julie Hall Anne-Cécile Déclais Arno Alpi David M. J. Lilley Shawn Ahmed Anton Gartner

DNA double-strand breaks (DSBs) can be repaired by homologous recombination (HR), which can involve Holliday junction (HJ) intermediates that are ultimately resolved by nucleolytic enzymes. An N-terminal fragment of human GEN1 has recently been shown to act as a Holliday junction resolvase, but little is known about the role of GEN-1 in vivo. Holliday junction resolution signifies the completio...

2015

Single-strand break repair and genetic disease. DNA single-strand break repair SSBR is critical for the survival and. The association of human genetic disorders with defects in the DNA.encounter DNA single-strand breaks or other types of lesion. An inability to respond properly to, or to repair, DNA damage leads to genetic instability. Developmental disorders, Werner syndrome WS and Bloom syndr...

Journal: :Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2015
Songliu Hu Songbin Fu Xiangying Xu Lin Chen Jianyu Xu Bin Li Yuanyuan Qu Hongyang Yu Shan Lu Wenxin Li

BACKGROUND/AIMS Survivin, a member of the inhibitor of apoptosis protein family, is an attractive target for cancer therapy. We investigated the effects of YM155, a small molecule inhibitor of survivin expression, on the radiosensitivity of human non-small cell lung cancer (NSCLC) cell lines and elucidated a relationship between the cellular localization of survivin and DNA double-strand break ...

Journal: :Cancer research 2005
Harm H Kampinga Andrei Laszlo

In their recent article, Takahashi et al. (1) suggest that heatinduced DNA double strand breaks contribute to heat-induced cell killing because heat treatment induces histone gH2AX-containing foci. Such foci have been associated with double strand breaks induced by ionizing radiation, other agents, and other stresses (2). However, the authors disregard the hyperthermia biology literature, which...

2017
Elvira Manjón Tomás Edreira Sofía Muñoz Yolanda Sánchez

Rho GTPases are conserved molecules that control cytoskeletal dynamics. These functions are expedited by Rho GEFs that stimulate the release of GDP to enable GTP binding, thereby allowing Rho proteins to initiate intracellular signaling. How Rho GEFs and Rho GTPases protect cells from DNA damage is unknown. Here, we explore the extreme sensitivity of a deletion mutation in the Rho1p exchange fa...

Journal: :The EMBO journal 2013
Soo-Hyun Yang Ruobo Zhou Judith Campbell Junjie Chen Taekjip Ha Tanya T Paull

The human SSB homologue 1 (hSSB1) has been shown to facilitate homologous recombination and double-strand break signalling in human cells. Here, we compare the DNA-binding properties of the SOSS1 complex, containing SSB1, with Replication Protein A (RPA), the primary single-strand DNA (ssDNA) binding complex in eukaryotes. Ensemble and single-molecule approaches show that SOSS1 binds ssDNA with...

2014
Masahiro Terasawa Akira Shinohara Miki Shinohara

Double-strand breaks (DSBs) are one of the severest types of DNA damage. Unrepaired DSBs easily induce cell death and chromosome aberrations. To maintain genomic stability, cells have checkpoint and DSB repair systems to respond to DNA damage throughout most of the cell cycle. The failure of this process often results in apoptosis or genomic instability, such as aneuploidy, deletion, or translo...

Journal: :Genomics 2009
Deepa Srikanta Shurjo K Sen Charles T Huang Erin M Conlin Ryan M Rhodes Mark A Batzer

The Alu family is a highly successful group of non-LTR retrotransposons ubiquitously found in primate genomes. Similar to the L1 retrotransposon family, Alu elements integrate primarily through an endonuclease-dependent mechanism termed target site-primed reverse transcription (TPRT). Recent studies have suggested that, in addition to TPRT, L1 elements occasionally utilize an alternative endonu...

Journal: :Asian journal of andrology 2007
Nicole Baumer Marie-Luise Sandstede Sven Diederichs Gabriele Kohler Carol Readhead Ping Ji Feng Zhang Etmar Bulk Jorg Gromoll Wolfgang E Berdel Hubert Serve Carsten Muller-Tidow

AIM To analyze the functional interactions of Cyclin with p53 and Atm in spermatogenesis and DNA double-strand break repair. METHODS Two lines of double knockout mice were generated. Spermatogenesis and double strand break repair mechanisms were analyzed in Cyclin A1 (Ccna1); p53- and Ccna1; Atm-double knockout mice. RESULTS The block in spermatogenesis observed in Cyclin A1-/- (Ccna1-/-) t...

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