نتایج جستجو برای: dipicolinic acid

تعداد نتایج: 747447  

Journal: :Applied and environmental microbiology 2015
Erwin M Berendsen Antonina O Krawczyk Verena Klaus Anne de Jong Jos Boekhorst Robyn T Eijlander Oscar P Kuipers Marjon H J Wells-Bennik

High-level heat resistance of spores of Bacillus thermoamylovorans poses challenges to the food industry, as industrial sterilization processes may not inactivate such spores, resulting in food spoilage upon germination and outgrowth. In this study, the germination and heat resistance properties of spores of four food-spoiling isolates were determined. Flow cytometry counts of spores were much ...

Journal: :Applied and environmental microbiology 2005
Remco Kort Andrea C O'Brien Ivo H M van Stokkum Suus J C M Oomes Wim Crielaard Klaas J Hellingwerf Stanley Brul

This study is aimed at the development and application of a convenient and rapid optical assay to monitor the wet-heat resistance of bacterial endospores occurring in food samples. We tested the feasibility of measuring the release of the abundant spore component dipicolinic acid (DPA) as a probe for heat inactivation. Spores were isolated from the laboratory type strain Bacillus subtilis 168 a...

Journal: :The Biochemical journal 2004
Jing Wu Mayur A Patel Appavu K Sundaram Ronald W Woodard

An open reading frame, encoding for KDOPS (3-deoxy-D-manno-octulosonate 8-phosphate synthase), from Arabidopsis thaliana was cloned into a T7-driven expression vector. The protein was overexpressed in Escherichia coli and purified to homogeneity. Recombinant A. thaliana KDOPS, in solution, displays an apparent molecular mass of 76 kDa and a subunit molecular mass of 31.519 kDa. Unlike previousl...

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