AIM The aim of the present study was to systematically review the influence of orthodontic force on human dental pulp. METHODS AND RESULTS The addressed focused question was "Do orthodontic forces affect the human dental pulp?" which was based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, a specific question was constructed according to the PIC...

The dental pulp is a sterile highly vascularized tissue and has been commonly used as a biological material to detect the genome of infectious agents that reach the dental tissue. Indeed, the pulp is also used to reveal past and ancient infections in the field of paleomicrobiology. The present study aimed to detect the presence of Hepatitis C virus (HCV) in a small community (approximately 400 ...

AIM To investigate the ability of human amniotic fluid stem cells and human dental pulp stem cells to differentiate into insulin-producing cells. METHODS Human amniotic fluid stem cells and human dental pulp stem cells were induced to differentiate into pancreatic β-cells by a multistep protocol. Islet-like structures were assessed in differentiated human amniotic fluid stem cells and human d...

considering the ease of isolation and high expansion potential of pulp stem/progenitor cells isolated from wisdom and primary teeth they have been implicated as the most reliable autologous cell source in dental tissue engineering. meanwhile, different isolation methods have remarkable impacts on the expansion potential of adult stem cells. in enzymatic digestion method extracted teeth as denta...

OBJECTIVE To assess if injection of allogeneic dental pulp tissue particles would improve lameness in horses with naturally occurring osteoarthritis (OA) or soft tissue (ST) injury. DESIGN Prospective, randomized, blinded, and controlled clinical trial and client survey assessment. ANIMALS Forty lame client-owned horses. PROCEDURES Sterile dental pulp, recovered from otherwise healthy foa...

Background: Dental caries is a bacterial disease affecting the hard tissue of the teeth as well as the pulp. The human dental pulp consists of odontoblast which are organized as a densely packed cell layer. Odontoblasts is located at the periphery of the pulp; therefore, they are the first cells encountered by cariogenic bacteria and their products that are represented in the carious lesion. Pu...

Dental pulp stem cells (DPSCs) are a promising cell source for regeneration of dental pulp. Migration is key event but influence the microenvironment rigidity (5 kPa at center to 20 GPa dentin) largely unknown. Mechanical signals transmitted from extracellular matrix cytoskeleton, nuclei, and chromatin, potentially regulating gene expression. To identify microenvironmental on migration, we anal...

The isolation of human genomic DNA is an important step involved in genetic identity analysis. Also getting optimal quantity and quality of DNA is important as this can alter the results. This consideration is especially important in the field of forensic analysis and genetic identity, where DNA is extracted from extremely small amounts of starting material often recovered from suboptimal stora...

background: the use of electric current during application of etch-and-rinse adhesive systems has been recently introduced to decrease microleakage. this study investigated the effects of an electric field produced by an experimental device for the application of a two-step etch-and-rinse adhesive on moist dentin surface. methods: sixty freshly extracted human premolars were used for this study...

INTRODUCTION Dental pulp has neural fibers that produce neuropeptides like Substance P (SP) and calcitonin gene-related peptide (CGRP). The inflammation of dental pulp can lead to an increase amount of SP and CGRP release, especially in symptomatic irreversible pulpitis. Therefore, it can be assumed that neuropeptides have some role in the progression of inflammation of the dental pulp. The aim...