Rotationally resolved S S fluorescence excitation spectra of 2-methyl anisole (2MA) and 3-methyl anisole (3MA) have been obtained in the environment of a supersonic jet environment. The derived values of the rotational constants show that 2MA itself has a planar heavy-atom structure with trans-disposed methoxy groups in both electronic states. No evidence for any other conformational isomers wa...

Crystalline bovine or porcine glucagon was fractionated by chromatography on columns of DEAE-cellulose in the presence of buffers containing ‘7 M urea. About 89% of the recovered protein comprises highly purified glucagon; 10% is monodesamidoglucagon and another 0.8 % resembles glucagon in amino acid composition and in electrophoretic mobility. The monodesamidoglucagon was identified and charac...

Cell ghosts were obtained from Candida utilis and Saccharomyces cerevisiae by the action of some conformational isomers of pancreatic ribonuclease which passed through the cell wall and penetrated the cell membrane. In the interior, the enzyme caused extensive disorganization of the cellular structure as evidenced by the results of vital staining and electron microscopy. Ribonucleic acid was de...

Phthiobuzone is a bis(thiosemicarbazone) derivative with a single chiral center which has been used as a racemate in the clinical treatment of herpes and trachoma diseases. In this study, its two enantiomers were prepared from chiral amino acids and their absolute configurations were investigated by electronic circular dichroism (ECD) combined with modern quantum-chemical calculations using tim...

An enantioselective and diastereoselective approach toward the synthesis of the tetracyclic scaffold of the furanobutenolide-derived polycyclic norditerpenoids is described. Focusing on synthetic efforts toward ineleganolide, the synthetic approach utilizes a palladium-catalyzed enantioselective allylic alkylation for the construction of the requisite chiral tertiary ether. A diastereoselective...

New antibiotics, miyakamides A1, A2, B1, and B2, were isolated from the cultured broth of Aspergillus flavus Link var. columnaris FKI-0739 together with known compounds, parasiticolide A, hydroxyaspergillic acid, and kojic acid. The structure of miyakamide A1 is N-acetyl-L-phenylalanyl-N-methyl-L-phenylalanyl-(alphaZ)-alpha,beta-didehydrotryptamine, and miyakamide A2 is E isomer of A1 at didehy...

An understanding of the photoisomerization mechanism of molecules bound to a metal surface at the molecular scale is required for designing photoswitches at surfaces. It has remained a challenge to correlate the surface structure and isomerization of photoswitches at ambient conditions. Herein, the photoisomerization of a self-assembled monolayer of azobenzene-thiol molecules on a Au surface wa...

The funnel landscape model predicts that protein folding proceeds through multiple kinetic pathways. Experimental evidence is presented for more than one such pathway in the folding dynamics of a globular protein, cytochrome c. After photodissociation of CO from the partially denatured ferrous protein, fast time-resolved CD spectroscopy shows a submillisecond folding process that is complete in...

New convulsive compounds, brasiliamides A (1) and B (2), were isolated by activity-guided fractionation from okara fermented with a soil isolate of Penicillium brasilianum Batista JV-379. Their structures were elucidated on the basis of spectral and chemical evidence and by X-ray crystallography of the hydrogenated product of 2. In the 1H- and 13C-NMR spectra of 2, the signals were complicated,...

Polyfluorenes reveal a complex interplay between emissive properties and intraand intermolecular structure. Vibrational frequencies and intensities determined by Raman spectroscopy are strongly influenced by variations in the backbone as well as side chain conformations. Changes in the structural and electronic properties of two side group substituted polyfluorenes (PF): ethyl-hexyl substituted...