Sertoli cells obtained from prepubertal rat testes were cultured in the presence or absence of retinol. Incorporation of monosaccharides and glycopeptide composition of the cells were studied under two experimental conditions. The results indicate that retinol increases the amount of mannose and glucosamine incorporated into cellular glycoconjugates. The labeled glycopeptides obtained from cont...

Cell wall mannoprotein of nonpathogenic yeasts is surface exposed, since the cells are agglutinated by concanavalin A and antimannoprotein antibodies. However, nonencapsulated cells of Cryptococcus neoformans were agglutinated neither by concanavalin A nor by antimannoprotein antibodies. Immunogold electron microscopy located most mannoprotein in the inner cell wall. Chemical analysis of purifi...

Objective(s): Interleukin-36 receptor antagonist (IL-36Ra) is a new member of the IL-1 family that exhibits anti-inflammatory activity in a variety of inflammatory and immune diseases. Our purpose was to determine the effect of IL-36Ra on liver injury in a mouse hepatitis model induced by concanavalin A (ConA). Materials and Methods: Mic...

Synaptic plasma membranes, synaptic junctions, and postsynaptic densities have been isolated from rat brain, the proteins resolved by polyacrylamide gel electrophoresis, and the glycoproteins identified. The synaptic junction is composed of a spectrum of polypeptides which range in Mr from 13,000 to 250,000. The overall pattern is similar to synaptic plasma memranes; however, the relative propo...

outer surface, similar protrusion inwards might be expected with isolated walls, whereas a difference in the gel structure of the wall could explain the morphological difference, and also the finding (Burger, 1966) that isolated walls bind little more antibody than do whole bacteria. The inacessibility of new teichoic acid to concanavalin A at the bacterial surface shows that the teichoic acid ...

Concanavalin A yielded a monodiperse pattern in the analytical ultracentrifuge in the pH range 2-5. The sedimentation coefficient, sOZ~,~ in pH 5 acetate buffer containing 0.1 M NaCl (total I’/2 = 0.20) was 4s. At pH 7 and above, a two-peak schlieren pattern was observed, the faster component (about 7s) probably representing a dimer. On the basis of an s~o.~ of 3.9s; a D 20.~ of 5.43 X 10-T cm2...

Deposition of behenic acid (B.A) mono and bilayers onto gold coated surfaces was performed by Langmuir-Blodget dip-casting technique. Surface Plasmon resonance (SPR) and ellipsometry methods were employed for investigation of the monolayer and bilayer films. The adsorption of the biologically important molecule Concanavalin A (Con A) from bulk solution to these monolayers and bilayers as we...

A ~ t T h e interaction of concanavalin A with a comprehensive series of carbohydrate-bovine serum albumin conjugates was investigated by agar gel diffusion and quantitative precipitation techniques. These studies showed that when conjugated to bovine serum albumin, those sugars which inhibited concanavalin A-polysaccharide interaction formed a precipitate with concanavalin A. The unexpected re...