Coagulants can be extracted from vegetal material and applied in the treatment of wastewater. These coagulants are derived seeds, leaves, bark, roots fruits. This study focuses on use mucilaginous extract Stenocereus griseus (known as Cardón Guajiro) for removal biochemical oxygen demand (BOD5), chemical (COD), total solids, turbidity color domestic wastewater a pumping station city Cartagena (...

In a Dutch project for harmonisation of factor VIII coagulant activity (FVIII:C) assays, the commutability of potential calibrators for FVIII:C was assessed by means of a 'twin-study design', which is in essence a multi-centre, split-patient sample, between-field-methods protocol. Commutability was defined as the degree to which a material yielded the same numerical relationships between result...

Sedimentation analysis of factor VIII complex was performed in the analytical ultracentrifuge using partition cells. This method allowed for the calculation of three different sedimentation coefficients from each run: one based on ristocetin agglutination activity for von Willebrand protein. 5WF; one based on coagulant activity for factor VIIIC. and one based on the schlieren or adsorption data...

A recombinant Factor VIII (Factor VIII-delta II) consists of a unique polypeptide chain of 165 kDa deleted from the major part of the B-domain and from the cleavage site at Arg-1648-Glu-1649 found in plasma-derived Factor VIII. It was expressed in mammalian cells in serum-free medium containing von Willebrand factor and purified by a one-step immunopurification. The recombinant Factor VIII was ...