There are no generally accepted regimens for the treatment of H. pylori infection in patients with gastritis or duodenal ulcers. However, metronidazole based regimens have been reported to be among the most successful. Resistance to metronidazole, clarithromycin, and amoxicillin was determined for 46 clinical isolates of Helicobacter pylori in Saudi Arabia and tested by E test. Of these isolate...

Resistance to clarithromycin in breakthrough Mycobacterium avium complex (MAC) isolates typically occurs 3 to 4 months after the initiation of monotherapy in bacteremic AIDS patients. It has been suggested that continuation of clarithromycin therapy still results in clinical and microbiological improvement. To study this paradox, C57BL/6 beige mice were infected with a clarithromycin-resistant ...

In this study, two identical copies of a 23S-5S gene cluster, which are separately situated within the Helicobacter pylori UA802 chromosome, were cloned and sequenced. Comparison of the DNA sequence of the H. pylori 23S rRNA gene with known sequences of other bacterial 23S rRNA genes indicated that the H. pylori UA802 23S rRNA genes are closely related to those of Campylobacter spp. and therefo...

BACKGROUND The increasing use of macrolides especially in the treatment of Helicobacter pylori infection has led to an increase in resistant strains. The resistance of H pylori to macrolides, especially clarithromycin, is one of the major causes of eradication failure. In H pylori, clarithromycin resistance is due to point mutations localised in domain V of 23S rRNA. AIM To develop a molecula...

Objective. To study primary resistance of H. pylori to clarithromycin in residents Gomel region by real-time polymerase chain reaction (PCR RT) using TaqMan® MGB probes. Materials and methods. The included 184 patients diagnosed with gastritis duodenitis, K29, median age 48.5 years (25% 75% were 37 61 old). According the patients’ questionnaires, no clarithromycin-based eradication therapy was ...

objective(s): macrolide resistant streptococcus pneumoniae pose an emerging problem globally. the aim of this study was to investigate the prevalence of ermb and mefa genes (macrolide resistant genes) by polymerase chain reaction (pcr) method and to detect drug resistance patterns of s. pneumoniae isolated from clinical samples to macrolides and other antibiotic agents by e-test method. materia...

A commercially available rapid fluorescent in situ hybridization (FISH) test, (seaFAST H. pylori Combi-Kit; SeaPro Theranostics International, Lelystad, The Netherlands) was used to simultaneously detect the presence of Helicobacter pylori and determine clarithromycin susceptibility in paraffin-embedded biopsy sections. The FISH method was found to be 97% sensitive, 94% specific for the detecti...

We read with interest the recently published paper by Piccolomini et al. (7). They evaluated three different methods to study the susceptibility of Helicobacter pylori to 20 antimicrobial agents. They found a very good correlation between E-test and agar dilution methods, as well as between broth microdilution and agar dilution. They recommended the E test as a reliable and alternative method f...

Introduction: The importance of H. pylori as an etiological agent in gastroduodenal disease had suggested antibiotic treatment as a main target for the elimination of infection. The successful eradication of H. pylori infection was shown to resolve the gastritis, dramatically accelerate ulcer healing, reduce ulcer recurrence and the prophylactic effect on the recurrence of ulcer bleeding. Mater...

Antimicrobial susceptibility testing was performed on 48 isolates of Helicobacter pylori recovered from Egyptian children undergoing routine endoscopies. The isolates were universally highly resistant to metronidazole, but resistance to other tested antimicrobial agents was rare (4% for clarithromycin, erythromycin, and azithromycin resistance versus 2% for ciprofloxacin and ampicillin resistan...