Lipopeptides are currently re-emerging as an interesting subgroup in the peptide research field, having historical applications as antibacterial and antifungal agents and new potential applications as antiviral, antitumor, immune-modulating and cell-penetrating compounds. However, due to their specific structure, chromatographic analysis often requires special buffer systems or the use of trifl...

The nitrate-selective copperised-cadmium (Cu-Cd) reduction reaction coupled directly to the highly sensitive nitrite ion chromatographic detection, produced a more precise method for determination of nitrate than any one of the two conventional methods. A borate buffer solution used in the reduction reaction, in place of the conventional ammonium-EDTA buffer solution, eliminated interferences f...

the purification of biomolecules is a necessary step in many biochemical researches. in this regard, developments of convenient, specific and low cost methods of purification are of particular interest. given the human hemoglobin (hb) affinity toward some charged carbohydrates, interaction of this molecule with human chorionic gonadotropin (hcg) which is a glycoprotein hormone containing sialic...

A sensitive, stability-indicating reversed-phase high-performance liquid chromatographic method is developed and validated for the quantitative determination of retapamulin in topical dosage form. The chromatographic separation is achieved by using a C18 column (XTerra RP 18 250 × 4.6 mm, 5 µm) at 30°C. The mobile phase comprises a mixture of 0.05M potassium dihydrogen phosphate buffer (pH 6.1)...

Objective: The present aim is to develop simple, precise, and accurate reverse phase high performance liquid chromatographic method (RP-HPLC) for the simultaneous assay of artemether and lumefantrine in fixed combined dosage form. Methods: The chromatographic study was carried out on Hypersil C18 column (250×4.6 mm, 5 μ) with mobile phase containing a mixture of KH2PO4 buffer (pH-3.5) and aceto...

A "high-performance" liquid-chromatographic procedure for 5-hydroxy-3-indoleacetic acid is described and compared with a colorimetric method in which 1-nitroso-2-naphthol is used. The analyte and an internal standard, p-nitrobenzoic acid, were extracted into diethyl ether from urine at pH 4.0 (acidified with HCl) to which sodium chloride had been added, and the ether was back-extracted with ace...

An isocratic RP-HPLC method (Method-A) and two visible spectrophotometric methods (Method B and C) were developed and validated for the determination of Clobazam in bulk and tablet dosage forms. In Method-A, a PEAK chromatographic system equipped with Zodiac C-18 (250mm, 4.6mm and 5μm) column, mobile phase of composition 10:30:60 (v/v/v) of tetra hydro furan (THF), methanol (MeOH) and acetonitr...

An isocratic liquid chromatographic method with UV detection at 220 nm is described for simultaneous determination of ceftriaxone sodium and sulbactam sodium in Sulbactomax. Chromatographic separation of two drugs was achieved on a Hypersil ODS C-18 column using a mobile phase consisting of a binary mixture of acetonitrile and tetrabutyl ammonium hydroxide adjusted to pH7.0 with orthophosphoric...

An environmentally friendly, facile and solvent-free synthetic route for aluminum sulfate octadecahydrate (Al2(SO4)3.18H2O) catalyzed one-pot multi-component synthesis of 12-aryltetrahydrobenzo[α]xanthene-11-ones, 1,8-dioxo-octahydroxanthenes and 14-aryl-14Hdibenzo[α,j]xanthenes has been developed. The readily, easy to handle, non-toxic, environmental friendly and low-cost catalyst, easily sepa...