in this study the effect of the alkylating agent, sulfur mustard, on calf thymus chromatin was investigated using uv/vis spectroscopy, gel electrophoresis and thermal denaturation techniques. the results show that treatment of isolated chromatin with sulfur mustard releases histones from the core particles but does not affect histone h i and nonhistone chromosomal proteins. the content of relea...

DNA's from diverse cells of different species and from diverse tissues give the same x-ray diffraction pattern. The presently observable structure of DNA appears, then, to be the same in all cells. Thus, DNA in the resting state-the stored genetic material, from sperm of Paracentrotus lividus, Arbacia lixula, and salmon and from T(2) and T(7) bacteriophage-gives a pattern indistinguishable from...

Dithiocarbamates, with their high lipophilic character and good chelating properties, could provide stabile transition metal complexes enable these metal-based drugs to reach biological targets. Palladium(II) gold(III) complexes, due its structural similarity platinum(II)compounds, be competitive candidates for implementing developing new pharmacological agents. Herein, novel complex triammoniu...

A novel ternary Schiff base ligand (HL) of ONO type and its copper (II) complex weresynthesised using 2-aminophenol and o-acetoacetotoluidide. They have been characterised by theusual analytical and spectral methods. The interaction of the complex with calf-thymus (CT)DNA has been investigated by UV-Vis, viscosity measurement, cyclic voltammetry anddifferential pulse voltammetry studies. The re...

The experimental determination of formation constant for interaction of two water soluble phthalocyanines, Cobalt (II) 4,4',4",4'"-tetrasulpho phthalocyanine (CoTsPc ) and Manganese(II) 4,4',4",4"-tetrasulpho phthalocyanine (MnTsPc ) , with calf thymus DNA have been studied by UV-Vis spectrophotometric method at lrnM phosphate buffer , pH 7.4 and at 5 different temperatures 20,25,30,35 and 400 ...

Strong electrostatic interaction between metallo-supramolecular polymers and DNA was confirmed by UV-vis and CD spectral measurements during titration, and cyclic voltammetry. The stable conjugation structure based on groove binding was revealed by using QM/MM computational methodology and supported by AFM.

We study the rheology of semi-dilute solutions of the sodium salt of calf-thymus DNA in the linear and nonlinear regimes. The frequency response data can be fitted very well to the hybrid model with two dominant relaxation times τ0 and τ1. The ratio (τ0=τ1) 5 is seen to be fairly constant on changing the temperature from 20 to 30C. The shear rate dependence of viscosity can be fitted to the Car...

Five major histone proteins have been extracted from chromatin isolated from purified nuclei of the fungus, Aspergillus nidulans. These proteins had chromatographic properties which were similar to reference calf thymus histones and were purified to electrophoretic homegeneity by gel chromatography of Bio-Gel P10, Bio-Gel P60, and Sephadex G-100. Electrophoresis of these proteins in three diffe...

The F3 histones of duck erythrocytes and calf thymus have been purified and compared by a variety of techniques. The results of analytical gel electrophoresis, gel filtration, cysteine content determinations, and amino acid analysis support the conclusion that there exist two chemically and metabolically distinct forms of F3 histone. The duck erythrocyte has a single form of F3 histone, which c...

The interactions of four antitumor azolato-bridged dinuclear platinum(II) complexes, [{cis-Pt(NH(3))(2)}(2)(μ-OH)(μ-azolato)](2+), with calf thymus DNA were monitored dose- and time-dependently, by using circular dichroism. Complexes 1-4 reacted with DNA via a two-step interaction that comprised a prompt diffusion-controlled reaction, which induced a B- to C-form transition, and a relatively sl...