The antifungal potential of volatile organic compounds (VOCs) produced by actinobacterial strains Streptomyces sp. (ACTB-77) and Amycolatopsis (ACTB-290) from the rhizosphere Caatinga plants against Colletotrichum musae was investigated. VOCs these microorganisms (axenic co-culture) were investigated using headspace-solid phase microextraction (HS-SPME) gas chromatography-mass spectrometry (GC-...

Marine-based biomolecules are emerging metabolites that have gained attention for developing novel biomaterials, drugs, and pharmaceutical in vitro platforms. Here, we developed a 3D engineered neural co-culture model via prototyped sliding frame-platform multi-step UV lithography investigated the neurovascular potential of citreohybridonol neuroblastoma treatment. Citreohybridonol was isolated...

Two bacterial isolates were obtained in axenic culture from the rhizosphere soil of Astragalus bisulcatus, a legume able to hyperaccumulate selenium. Both strains resulted of particular interest for their high resistance to the toxic oxyanion SeO3(2-) (selenite, Se(IV)). On the basis of molecular and biochemical analyses, these two isolates were attributed to the species Bacillus mycoides and S...

The axenization of an Entamoeba histolytica isolate with a nonpathogenic isoenzyme electrophoretic pattern (zymodeme) was recently achieved for the first time (15). Forty days after the cells were transferred to the medium used for axenic cultivation, the amebae developed virulence properties, and the zymodeme converted to a pathogenic pattern. To exclude the possibility that the original isola...

Microalgae have several applications in nutraceuticals, pharmaceuticals, cosmetics, biofuel production, and bioremediation, among other fields. Isolation purification are extremely important for obtaining axenic cultures of microalgae from different environments crucial their biotechnological applications, but it is not an easy task. In view the above, fundamental to know classical advanced tec...

b ackground: acanthamoeba spp . are free-living amoebae commonly found in the environmental sources such as water, soil, and air. this ubiquitous amoeba is the causative agent of amoebic kerati- tis (ak). the objective of the present study was to investigate the presence of acanthamoeba spp. in water and soil sources in ahvaz city, khuzestan province, southern iran. m ethods: in general, 110 sa...

Background and Aims: In most of the studies, Toxoplasma gondii is maintained in laboratory mice or studied in vitro using non-lymphoid cell lines or primary mouse macrophages. The target of our research was to design a new axenic culture of Toxoplasma gondii tachyzoites to providing a sufficient quantity of them. Material and Methods: Theileria annulata-infected lymphoblastoids, which had been...