The interactions of HIV-1 reverse transcriptase (HIV-1 RT) with a synthetic 53/19-mer DNA substrate was investigated. For this template-primer HIV-1 RT displayed a Km value of 20 nM. The 53/19-mer competitively inhibited DNA synthesis performed on poly (rC).oligo(dG) with Ki value of 260 nM. This corresponded well to an equilibrium dissociation constant (Kd) of 300 nM, as determined by analytic...

the matter of meaning in place, is one of the main qualities of human life. people consciously or unconsciously looking for meanings in places. the importance of finding the meaning of place is that, understanding the meaning will lead to “act” in place. finding the place friendly, or finding it insecure will lead to act differently. now the question is that, is the meaning of place, something ...

Association constants were determined for absorption of the sex-specific agglutinin from mating type 5 of Hansenula wingei by cells of type 21, the opposite mating type. Absorption was determined by following the radiolabeled sulfur in purified agglutinin extracted from cells grown in 35S-labeled medium. Association was proved to be reversible under conditions where cell agglutination was In se...

BACKGROUND Alpha1-antitrypsin and surfactant protein-A (SP-A) are major lung defense proteins. With the hypothesis that SP-A could bind alpha1-antitrypsin, we designed a series of in vitro experiments aimed at investigating the nature and consequences of such an interaction. METHODS AND RESULTS At an alpha1-antitrypsin:SP-A molar ratio of 1:1, the interaction resulted in a calcium-dependent d...

most of the biological phenomena are influenced by intermolecular recognition and interaction. thus, understanding the thermodynamics of biomacromolecule ligand interaction is a very interesting area in biochemistry and biotechnology. one of the most powerful techniques to obtain precise information about the energetics of (bio) molecules binding to other biological macromolecules is isothermal...

Structural determinants of oligosaccharides affecting interaction with the saccharide-binding sites of two lectins, the agglutinin of M, 120,000 and toxin of 82, 60,000 (RCA* and RCA~I, respectively), isolated from Ricinus communis beans have been examined by performing binding studies with iodinated glycopeptides and their degradation products. Association constants can be determined by Scatch...