Tumor necrosis factor receptor 1 (TNFR1) activates NF-?B–dependent pro-inflammatory gene expression, but also induces cell death by triggering apoptosis and necroptosis. Inhibition of inhibitor NF-?B kinase (IKK)/NF-?B signaling in keratinocytes paradoxically unleashed spontaneous TNFR1-mediated skin inflammation mice, the underlying mechanisms remain poorly understood. Here, we show that TNFR1...

Background/Aim: Non-small cell lung cancer patients with epidermal growth factor receptor (EGFR) mutation have been shown to a good response erlotinib, tyrosine kinase inhibitor of EGFR. In this study, we found that the death pathways activated by erlotinib in 2D and 3D culture systems are different. Materials Methods: The induced were evaluated flow cytometry immunoblotting both EGFR mutant ce...

Apoptosis is a genetically programmed series of events that results in cell death. As a consequence, it is difficult to identify dominant genes that play a role in this process using genetic selections in conventional cell culture systems. Accordingly, we have established an efficient expression screen to isolate dominant, apoptosis-inducing genes. The assay is based on the apoptotic morphology...