Hydralazine, a widely used therapy for hypertension and heart failure, can elicit autoimmune disease, including anti-neutrophil cytoplasmic antibody associated glomerulonephritis (ANCA-GN). We identified 80 cases of ANCA-GN complicating treatment with hydralazine, accounting 4.3% (80/1858 biopsies) diagnosed between 2006 2019. Over three-fourths patients were on hydralazine at least one year, m...

The existence of cytoplasmic estrogen receptor immunoreactivity (ER-IR) has been reported in the brain using three different antibodies raised against different epitopes on the ER protein. With each antibody, the darkest reaction product was seen within cell nuclei, but cytoplasmic ER-IR was also observed with each antibody in most neuroanatomical areas. We have reported previously that an inje...

Mice were immunized with 1 to 3 micrograms of cytoplasmic estrogen receptor fragment purified from human myometrium by affinity chromatography. Two RE-antibody-secreting clones were detected from one fusion that were capable of precipitating cytosol RE. Monoclonal antibody D5 (subclass IgG1) reacts with an antigen that is related to RE from immunoprecipitation studies but which can be separated...

Case. We report a rare case of hydralazine-induced anti-neutrophil cytoplasmic antibody-associated vasculitis. A 75-year-old African American woman with history of high blood pressure on hydralazine for 3 years presented with acute onset of shortness of breath and hemoptysis. Lab workup revealed a severe normocytic anemia and a serum creatinine of 5.09 mg/dL (baseline 0.9). Bronchoscopy demonst...