Plasmodium falciparum histidine-rich protein 2 (PfHRP 2) was selected in this work as the biomarker for the detection and diagnosis of malaria. An enzyme-linked immunosorbent assay (ELISA) was first developed to evaluate the immunoreagent's suitability for the sensor's development. A gold-based sensor with an integrated counter and an Ag/AgCl reference electrode was first selected and character...

An electrochemical immunosensor based on screen-printed gold working electrode with onboard carbon counter and silver-silver chloride pseudo-reference electrode for Salmonella typhimurium detection is described in this paper. Monoclonal anti-S. typhimurium antibody was immobilized using physical and covalent immobilization via amine coupling of carboxymethyldextran on the surface of the gold wo...

Abstract Purpose The glycolytic enzyme enolase plays important role in the pathogenesis of Candida albicans infection and has been also considered as a promising molecular marker for diagnosis invasive candidiasis. This study aimed to investigate location secretion features (CaEno) with couple specific monoclonal antibodies (mAbs). Methods Two mAbs named 9H8 10H8 against CaEno were generated by...

DNA microarrays were rapidly scaled up from 256 to 6.5 million targets, and although antibody microarrays were proposed earlier, sensitive multiplex sandwich assays have only been scaled up to a few tens of targets. Cross-reactivity, arising because detection antibodies are mixed, is a known weakness of multiplex sandwich assays that is mitigated by lengthy optimization. Here, we introduce (1) ...

This highly sensitive immunoenzymometnc assay method involves monoclonalantibodies, a common-capturemicrosphere, and a rapid, membrane-filtration separation step. The common-capture solid phase is monoclonal anti-fluorescain antibody covalently attached to 6.5-sm-diameter latex particles. In sandwich-type assays for large-molecule analytes, the capture antibody is conjugated with fluorescein Is...

A new and enzyme-free electrochemical immunoassay protocol was developed for the sensitive electronic monitoring of neuron-specific enolase (NSE) on a monoclonal mouse anti-human NSE antibody (mAb)-modified glassy carbon electrode, using guanine-decorated graphene nanostructures (GGN) as nanotags. To construct such an enzyme-free immunoassay format, guanine and polyclonal rabbit anti-human NSE ...

In this study, it was aimed to reveal some viruses that are a problem in Dahlia plants grown for ornamental parks and home gardens Selçuklu, Karatay Meram districts of Konya. For reason, the 2019 summer, leaf samples were taken from 152 showing symptoms similar virus disease such as mosaic, deformations, yellowing, blight on leaves stunted plants. Possible infections Cucumber mosaic (CMV), Toma...

Gerbera spp., which is in the Asteraceae family, most widely cultivated cut flower ornamental plant Türkiye after carnation. Approximately 74% of Türkiye's production met from Antalya province. Virus diseases are a problem cultivation as they significantly reduce yield and quality. In this study, was carried out to detect some virus seen areas province, surveys were commercial 2021-2022 season....

Zucchini yellow mosaic virus (ZYMV) causes serious damage to cucurbit crops worldwide and can be spread by aphids, mechanical injury, in seeds. With the popularization of grafting, use susceptible rootstock has increased risk ZYMV infection crops. In China, bottle gourd ( Lagenaria siceraria ) is a widely used grafted watermelon production. However, few resistant gourds are available commercial...