Oncogenic transformation is characterized by morphological changes resulting from alterations in actin dynamics and adhesive activities. Emerging evidence suggests that the protocadherin FAT4 acts as a tumor suppressor in humans, and reduced FAT4 gene expression has been reported in breast and lung cancers and melanoma. However, the mechanism controlling FAT4 gene expression is poorly understoo...

Actin is abundant in the nucleus and it is clear that nuclear actin has important functions. However, mystery surrounds the absence of classical actin filaments in the nucleus. To address this question, we investigated how polymerizing nuclear actin into persistent nuclear actin filaments affected transcription by RNA polymerase II. Nuclear filaments impaired nuclear actin dynamics by polymeriz...

Profilin-1, a member of the Profilin family, is ubiquitously expressed protein that controls actin polymerization in concentration-dependent manner. As mutations Profilin-1 gene have potential implications neurodegenerative disease progression, well-characterized anti-Profilin-1 antibodies would be beneficial to scientific community. In this study, we characterized sixteen commercial for...

Ubiquitously expressed β-actin and γ-actin isoforms play critical roles in most cellular processes; however, their unique contributions are not well understood. We generated whole-body β-actin-knockout (Actb(-/-)) mice and demonstrated that β-actin is required for early embryonic development. Lethality of Actb(-/-) embryos correlated with severe growth impairment and migration defects in β-acti...

The expression of three different actin genes in the sea urchin, Strongylocentrotus purpuratus, was monitored in embryos and adult tissues by using untranslated mRNA sequences as specific hybridization probes. Three distinct patterns of expression were found: muscle specific, embryo specific, and constitutive (i.e., present in all tissues examined). The actin genes encoding the muscle-specific ...

for gene transfer research and studies of cell differentiation and regulation of gene expression. Identification of the genomic structure of fish genes provides valuable sequence information and adds to understanding of their molecular evolution. We aimed at cloning rainbow trout regulatory sequences that direct expression of genes in skeletal muscle. To address this task, we chose α-actin, myo...

Actin is an abundant, highly expressed, and much conserved protein belonging to the actin–heat shock protein70–sugarkinase superfamily. The full-length messenger RNAs encoding actin were cloned and characterized from the plant-parasitic cyst nematodes Heterodera glycines and Globodera rostochiensis and from the free-living nematode Panagrellus redivivus. The actins from the plant-parasitic nema...

Actin and spectrin are important constituents of axonal cytoskeleton. Periodic actin-spectrin structures found in dendrites, initial segment axon, main axon. Actin-spectrin periodicity has been hypothesized to be manipulating the axon stability mechanical behavior. Several experimental computational studies have performed focusing on behavior actin, spectrin, network. However, most actin focus ...

Background and Aim: A great interest for determining the particular mechanisms underlying lipogenesis and adipogenesis has been raised among researchers in order to fight obesity. We aimed to investigate the gene expression of FAS and its role in regulation of lipogenesis and adipogenesis in visceral adipose tissues from obese and normal-weight subjects. materials and Methods: A total of.parti...