نتایج جستجو برای: 80 rapd primers were employed for pcr reactions
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Various organisms have been characterized by molecular methods, including fungi of the genus Cryptococcus. The purposes of this study were: to determine the discriminatory potential of the RAPD (Random Amplified Polymorphic DNA) primers, the pattern of similarity of the Cryptococcus species, and discuss their useful application in epidemiological studies. We analyzed 10 isolates of each specie/...
A comparison of random amplified polymorphic DNA (RAPD) was used to investigate genetic polymorphisms among 25 isolates of Giardia intestinalis and to assess the utility of RAPD for subtype detection and genealogical analysis. Using data obtained for six human and 19 animal-derived isolates in polymerase chain reactions using 13 different primers, phylogenetic trees were constructed and bootstr...
The present work reports successful DNA amplification of Pantoea agglomerans and Bacillus pumilus through Random Amplified Polymorphic DNA (RAPD). For this, template DNA was obtained without conventional DNA extraction. The procedure was as follows: cultures grown for 20 hours in 5 mL LB medium were centrifuged and the resulting preparation was suspended in TE buffer. After boiling, the cell su...
Identifícation of RAPD markers linked to bacterial blight resistance genes in Phaseolous vulgaris L
Genetic studies indicate that resistance to bacterial blight is different depending on the source of resistance may be determined by both major and minor genes (Mcelroy 1985, Musaana el al, 1993. ). RAPD markers obtained from PCR amplification of genomic DNA with random primers have been successfully used for tagging genes of interest in common bean and other crops. For seed, pod and leaf react...
fusarium verticillioides (f. verticillioides) is not only a primary pathogen of maize, but also can causedisease in other crops such as sorghum. pathogenicity is related to mycotoxin production such as fumonisin.in the present study, 24 isolates of f. verticillioides, which were previously identified by phenotype basedmethods, were re-identified using restriction fragment length polymorphism (r...
Random-amplified polymorphic DNA (RAPD) markers (8) are a powerful tool for genome analysis (7). Nevertheless, the difficulties to achieve a high pattern repeatability represent a major drawback for the routine implementation of RAPD markers. Amplification products obtained depend on various factors, such as the concentration of the reaction mixture constituents, the source of Taq DNA polymeras...
After conducting a roving survey in Chittoor district for the natural occurrence of Nomuraea rileyi, a few fungal infected and died (mummified) cadavers of Spodoptera litura and Bombyx mori were found and collected. With microscopic studies, the fungus was identified as Nomuraea rileyi and Beauveria bassiana. The molecular characterization of 7 isolates of N.rileyi was done by RAPD-PCR for stud...
Two pairs of diagnostic primers, IHm01-L/IHm01-H and IHm02-L/IHm02-H, for distinguishing the Chinese crude drug Oviductus Ranae from its substitutes were designed based on sequences of Cyt b gene fragment of the original animals of the drug and substitutes. Total DNAs were extracted from crude drugs purchased from five drugstores in different regions, as well as from original animals of the dru...
The random-amplified polymorphic DNA (RAPD) technique (5,7) is one of the most useful methods for species identification and studies on the genetic structure of populations of microand macro-parasites. This method generally provides numerous markers that must be cloned and labeled to be used as probes (1,4). These probes can be used to test the specificity and the polymorphism of the RAPD marke...
The genetic differences between praziquantel-resistant (R) and susceptible (S) strains of Schistosoma mansoni (Fallon & Doenhoff, 1994) were explored using RAPD and by cloning differentially expressed mRNAs by subtractive PCR. No differences between the 2 strains were detectable by RAPD using 41 different primers indicating that no major genomic rearrangements were present. Subtractive PCR gene...
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