نتایج جستجو برای: western blotting method
تعداد نتایج: 1799289 فیلتر نتایج به سال:
Background: VP4 protein is as spikes on rotavirus outer capsid shell which is responsible for virus attachment to the host. VP4 induces production of neutralizing antibodies which could be used for serotyping of different isolates. Methods: Simian rotavirus SA11 gene 4 cDNA was cloned into a cloning plasmid pDONRTM by recombination reaction using clonase II enzyme mix. The resulting clone was c...
infectious bursal disease virus (ibdv) is the causative agent of gumboro disease, an infectious disease of global economic importance in poultry. the expression of heterologous proteins in p.pastoris is fast, simple and inexpensive. in this study, vp2 encoding gene of classical d78 ibdv was amplified using reverse transcription (rt) polymerase chain reaction (pcr) and cloned into ppiczαa vector...
the objective of this study was to compare different antigen preparations to produce monoclonalantibodies against bovine leukaemia virus gp51su. the four antigen preparations for immunization ofbalb/c mice were: cl: blv-flk cell lysate, uf: a fraction of cl (between 30 and 100 kda), wvp: wholevirus particles and sp: with ion exchange chromatography, gp51su was semipurified. a total of ninesucce...
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