INTRODUCTION The prevalence of H. pylori varies with geographic locations. To date there are no epidemiological data on its prevalence in Cyprus; therefore, we determined the prevalence and molecular characteristics of H. pylori infection in Cypriot patients. METHODOLOGY DNA extracted from 103 gastric biopsies was analyzed for the presence of H. pylori by PCR using primers for ureA. H. pylori...

AIMS To determine the relation among the cytotoxin associated gene (cagA) and vacuolating cytotoxin gene (vacA) status of Helicobacter pylori isolates, the associated clinical diseases, and the severity and pattern of chronic gastritis. METHODS Helicobacter pylori was cultured from gastric biopsies obtained from dyspeptic patients. DNA was extracted from the isolates and the cagA and vacA sta...

A total of 300 gastric biopsy samples and 50 Helicobacter pylori isolates were collected from Ethiopian adult dyspeptic patients. The vacA and cagA genes were detected in 90 and 79% of biopsy specimens, respectively, and in 100 and 87% of clinical isolates, respectively. Both genes were detected in 84% of the gastric biopsy samples and in 87% of the clinical isolates. Among vacA genotypes, the ...

BACKGROUND Helicobacter pylori (H. pylori) infection is known to be associated with a spectrum of gastroduodenal diseases. We studied the association of H. pylori virulence markers cytotoxin-associated gene (cagA) and vacuolating associated cytotoxin gene (vacA) alleles in patients with non ulcer dyspepsia (NUD), gastric ulcer (GU), gastric carcinoma (GC) and duodenal ulcer (DU). METHODS H. p...

BACKGROUND The cytotoxin associated gene A (cagA), and the vacuolating cytotoxin gene A (vacA) of Helicobacter pylori have been associated to phenotypic characteristics of virulence. The objectives of this study were to detect the presence of cagA and to characterize the allelic variants of vacA in 63 strains of H. pylori isolated from colonized individuals with different clinical outcomes. M...

Polarized epithelial monolayers of Madin-Darby canine kidney (MDCK) cells were used to study the pathogenicity of Helicobater pylori, with an emphasis on the effect of VacA. The adherence of H. pylori to MDCK monolayers resulted in a decrease in trans-epithelial resistance (TER) across the cell monolayer. Isogenic vacA mutants did not lower the TER, demonstrating that the effect is strictly lin...

objective(s): helicobacter pylori, a human specific gastric pathogen is a causative agent of chronic active gastritis. the vacuolating cytotoxin (vaca) is an effective virulence factor involved in gastric injury. the aim of this study was to construct a recombinant protein containing antigenic region of vaca gene and determine its antigenicity.   materials and methods: the antigenic region of v...

background: helicobacter pylori strains have two classical virulence genes, the cytotoxinassociated a ( caga ) gene and the vacuolating cytotoxin a ( vaca ) gene, which are located in the cag pathogenicity island ( cag pai). serum immunoglobulin g (igg) antibodies to h. pylori , especially, the caga antigen may be a reliable marker for selection of dyspeptic patients for upper endoscopy. method...

background and objective: helicobacter pylori has several strains with different degrees of virulence. the aim of this study was to detect two major important virulence factors, caga/vaca genotypes, and to determine correlations among different caga/vaca genotypes and histological features of chronic gastritis in iranian patients. methods: in this cross- sectional study, gastric biopsy was take...