We have developed a quantitative method for methylation analysis of the p16 gene based on real-time methylation-specific PCR (MSP). Real-time MSP is sensitive enough to detect down to 10 genome equivalents of the methylated p16 sequence. Application of real-time MSP to DNA from tumor-derived cell lines revealed complete concordance with conventional MSP analysis. Quantitative data generated by ...

......................................................................................................................................... ix Chapter 1. General Introduction ..................................................................................................... 1 Chapter 2. Characterization of Rickettsial Infection in Amblyomma americanum Ticks (Acari: Ixodidae) by Quantitative Rea...

1. Besides miR-133a, miR-133b and miR-208a, there are several other muscle-specific miRNAs, such as miR-1, etc. Why only these miRNAs were used in this study? 2. P2: qRT-PCR is not quantitative real-time polymerase chain reaction, but quantitative reverse transcription-polymerase chain reaction. 3. P6: “To date, no housekeeping miRNAs have been established and validated to normalize for the miR...

Brucellosis is primarily a worldwide zoonotic disease caused by Brucella species. The genus Brucella contains highly infectious species that are classified as biological threat agents. In this regard, the identification of Brucella can be a time-consuming and labor-intensive process posing a real risk of laboratory-acquired infection to the laboratory staff. This stud...

conclusions it is suggested that determination of the target genotype by plasma subtyping for choosing the proper antiviral therapy is essential but may result in therapy failure. hcv genotyping in pbmc samples, along with plasma specimens, might be more beneficial. therefore determining the hcv genotype in pbmcs, before beginning the therapy is useful due to the possibility of occult infection...

conclusions: nested pcr as a sensitive chlamydia trachomatis detection test and endocervical specimens has been offered to detect this bacterium in spontaneous abortion. besides, c. trachomatis screening among pregnant women can be suggested to prevent abortion. results: the number of intercourses per week and history of miscarriage can be known as the risk factors of abortion. frequency of c. ...