This work aims at studying the optimization of an on-line capillary electrophoresis (CE)-based tryptic digestion methodology for the analysis of therapeutic polypeptides (PP). With this methodology, a mixture of surrogate peptide fragments and amino acid were produced on-line by trypsin cleavage (enzymatic digestion) and subsequently analyzed using the same capillary. The resulting automation o...

Natural stable isotopes ratios (δ13Ccarb and δ18Ocarb) of carbonates archived in the geological record are routinely used to reconstruct local global paleo temperatures secular evolution biogeochemical carbon cycle. The state-of-the-art technique, employed since mid 20th century, measure these isotopic starts with field sampling followed by several steps physical chemical laboratory preparation...

introduction echo-planar imaging (epi) is a group of fast data acquisition methods commonly used in fmri studies. it acquires multiple image lines in k-space after a single excitation, which leads to a very short scan time. a well-known problem with epi is that it is more sensitive to distortions due to the used encoding scheme. source of distortion is inhomogeneity in the static b0 field that ...

B. A. DEHORITY. 1960. Effect of starch on the digestion of cellulose in vitro and in vivo by rumen microorganisms. Jour. Animal Sci. 19 (4) : 1268-1269. KAMSTRA, L. D., A. L. MOXON AND 0. G. BENTLEY. 1958. The effect of stage of maturity and lignification on the digestion of cellulose in forage plants by rumen microorganisms in vitro. Jour. Animal Sci. 17 (1) : 199-208. LE FEVRE, C. F. AND L. D...

EPMcreate is a creativity technique for requirements elicitation based on a 16-step process. These steps suggest focusing on requirements related to every combination of two different target users or viewpoints. A series of experiments confirmed its feasibility; its applicability, both as individual and group technique; and its greater effectiveness than brainstorming. However, analysts involve...

Antivenoms are preparations of intact or fragmented (F(ab')2 or Fab) immunoglobulin G (IgG) used in human medicine to treat the severe envenomings resulting from the bites and stings of various animals, such as snakes, spiders, scorpions, or marine animals, or from the contact with poisonous plants. They are obtained by fractionating plasma collected from immunized horses or, less frequently, s...