Background: Acrylamid (AA) is found in carbohydraterich foods that are prepared at high temperatures, such as French fries and potato chips. The toxic effects of AA in male animals include degeneration of epithelial cells of seminiferous tubules, decreased number of sperm and elevation in abnormal spermatozoa. The aim of this study was to evaluate the detrimental effects of AA on sperm paramete...

Lactating rabbits (n=1335) were artificially inseminated to study the effect of the addition of caffeine on rabbit semen stored for up to 96 h. Concentration of 0-5 mM/l were tested. Whereas a concentration of 0.2 mM/l increased spermatozoa motility, higher concentration values adversely affected reproductive parameters. Spermatozoa stored at 18 degrees C for 72-96 h did not have the capacity t...

A major secretory protein of the human epididymis that is taken up by maturing spermatozoa is homologous to the leukocyte antigen CD52. The epididymis was shown to be the sole source of CD52 in seminal fluid, since CD52 could be detected in seminal plasma from sperm-containing ejaculates and not in ejaculates of vasectomized patients by Western blot analysis. The glycoprotein is not expressed i...

The molecular basis of many forms of male infertility is poorly defined. One area of research that has been studied intensely is the integrity of the DNA in the nucleus of mature ejaculated spermatozoa. It has been shown that, in men with abnormal sperm parameters, the DNA is more likely to possess strand breaks. However, how and why this DNA damage originates in certain males and how it may in...

TVRDÁ EVA, TUŠIMOVÁ EVA, ZBYŇOVSKÁ KATARÍNA, JAMBOR TOMÁŠ, LUKÁČ NORBERT. 2016. Protective Effects of α-tocopherol on the Activity and Antioxidant Profile of Bovine Spermatozoa Subjected to Ferrous Ascorbate-Induced Oxidative Stress. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 64(4): 1245–1255. As spermatozoa are highly vulnerable to oxidative stress development, in ...

The goal of this research is to conduct a theoretical investigation about the effect electroosmotic forces on swimming sperms throughout cervical canal. To imitate male semen with self-propulsive spermatozoa, hyperbolic tangent fluid used as base liquid. Swimming move inside ciliated canal and peristalsis occurs due walls. perturbation method solve controlling partial differential set equations...

Background: Epididymal sperm has been recovered with the aim of conserving potentially valuable genetics Materialss from post mortem males for subsequent usage in assisted reproduction technologies. Among the quality parameters of spermatozoa, motility and velocity patterns are sensitive for chemical and physical components of medium which they were diluted. Also close correlation between sperm...

The predictive value of sperm chromatin integrity for pregnancy outcome following in-vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) was studied in 24 men attending a university-based assisted reproductive techniques laboratory using the flow cytometric sperm chromatin structure assay (SCSA). The SCSA is a measure of the susceptibility of sperm DNA to low pH-induced denatu...

the effect of zinc nano-particles (zn- nano- complex) on bull sperm quality after freeze-thawing process studied. ejaculates collected from four holstein bulls twice a week. on the day of semen collection, four ejaculates were pooled and diluted with bioxcell extender containing 0, 10-6, 10-5, 10-4, 10-3, 10-2 molar of zinc nano-complex and frozen. after thawing, sperm motility and motion param...

Computer-assisted semen analysis (CASA) is an automated and objective method of evaluating structural (e.g. morphology) functional sperm parameters motility hyperactivation). Sperm hyperactivation essential for successful fertilization thus important aspect in determining the fertility potential a male. In current study, CASA was used standard comparison ability phosphate buffered saline (PBS),...