Single cycle reporter viruses that preserve the majority of the HIV-1 genome, long terminal repeat-promoted transcription and Rev-dependent structural protein expression are useful for investigating the viral life cycle. Reporter viruses that encode the viral proteins in cis in this way have been lacking for feline immunodeficiency virus (FIV), where the field has used genetically minimized tra...

Quantifying induced virion production from single proviruses is important for assessing the effects of HIV-1 latency reversal agents. Limiting dilution ex vivo cultures of resting CD4(+) T cells from 14 HIV-positive volunteers revealed that virion production after T-cell activation from individual proviruses varies by 10,000-fold to 100,000-fold. High-producing proviruses were associated with i...

We introduce a new measure of complexity of integer hulls of rational polyhedra called the small Chvátal rank (SCR). The SCR of an integer matrix A is the number of rounds of a Hilbert basis procedure needed to generate all normals of a sufficient set of inequalities to cut out the integer hulls of all polyhedra {x : Ax ≤ b} as b varies. The SCR of A is bounded above by the Chvátal rank of A an...

AIMS Premature ventricular complexes (PVCs) due to spontaneous calcium (Ca) release (SCR) events at the cell level can precipitate ventricular arrhythmias. However, the mechanistic link between SCRs and PVC formation remains incompletely understood. The aim of this study was to investigate the conditions under which delayed afterdepolarizations resulting from stochastic subcellular SCR events c...

Hepatitis C virus (HCV) entry, translation, replication, and assembly occur with defined kinetics in distinct subcellular compartments. It is unclear how HCV spatially and temporally regulates these events within the host cell to coordinate its infection. We have developed a single molecule RNA detection assay that facilitates the simultaneous visualization of HCV (+) and (-) RNA strands at the...

the purpose of this paper is multi-objective optimization of refrigeration cycle by optimization of all components of the cycle contains heat exchangers, air condenser, evaporator and super-heater. studied refrigeration cycle is compression refrigeration cycle of unit 132 third refineries in south pars that provide chilled water for cooling refinery equipment's. cycle will be performed by ...

Significance Studies on bacterial and eukaryotic viruses have revealed a range of strategies used by to subdue host cells for efficient virus replication. How archaeal interact with their hosts remains largely unknown. We characterize new strategy employed nonlytic STSV2 transform its into giant virion-producing factory, whereby the infection blocks normal cell division binary fission, leading ...

We have found that mycoplasma virus L172 is an enveloped globular virion containing circular, single-stranded DNA of 14.0 kilobases. L172 has been reported by other workers to have a double-stranded DNA genome of 13 to 17 kilobase pairs and has been classified as a plasmavirus, a group for which mycoplasma virus L2 is the type member. Mycoplasma viruses L172 and L2 differ in genome size and str...

We report the recording and reconstruction of x-ray diffraction patterns from single, unstained viruses, for the first time. By separating the diffraction pattern of the virus particles from that of their surroundings, we performed quantitative and high-contrast imaging of a single virion. The structure of the viral capsid inside a virion was visualized. This work opens the door for quantitativ...