نتایج جستجو برای: scatchard

تعداد نتایج: 1284  

Journal: :iranian journal of pharmaceutical research 0
somaye shahraki department of chemistry, university of sistan & baluchestan, zahedan, hassan mansouri torshizi department of chemistry, university of sistan & baluchestan, zahedan ziba sori nezami department of chemistry, university of sistan & baluchestan, zahedan, arezou ghahghaei department of biology, university of sistan & baluchestan, zahedan fateme yaghoubi department of chemistry, university of sistan & baluchestan, zahedan adeleh divsalar department of biological sciences, tarbiat moallem university, tehran

in depth interaction studies between calf thymus deoxyribonucleic acid (ct-dna) and a series of four structurally relative palladium(ii) complexes [pd(en)(hb)](no3)2 (a-d), where en is ethylenediamine and heterocyclic base (hb) is 2,2'-bipyridine (bpy, a); 1,10-phenanthroline (phen, b); dipyridoquinoxaline (dpq, c) and dipyridophenazine (dppz, d) (figure 1), were performed. these studies h...

Journal: :Plant physiology 1982
N Carrillo R H Vallejos

The binding of ferredoxin-NADP reductase to spinach chloroplast membranes was studied by washing the membranes with different media. Release of the enzyme from the thylakoids was greater in 0.75 millimolar EDTA but was not complete inasmuch as 20% the activity remained membrane-bound after three washes.A Scatchard plot of binding experiments suggests the presence of one type of binding site and...

2002
Laura Guida Qiyu Sun Cameron van den Heuvel David Gillis

Freshly isolated peripheral blood neutrophils, unlike monocytes and eosinophils, do not bind interleukin-3 (IL-3) or respond t o IL-3. We show that neutrophils cultured for 24 hours in granulocyte-macrophage colony-stimulating factor (GM-CSF) express mRNA for the IL-3 receptor (R) a subunit, as shown by RNase protection assays, and lL-3Rn chain protein, as shown by flow cytometric analysis usin...

2011
Chang-Chuan Guo Yi-Hong Tang Hai-Hong Hu Lu-Shan Yu Hui-Di Jiang Su Zeng

The protein binding of non-steroidal anti-inflammatory drugs flurbiprofen, ketoprofen and etodolac with human serum albumin (HSA) was investigated using indirect chiral high performance liquid chromatography (HPLC) and ultrafiltration techniques. S-(-)-1-(1-naphthyl)-ethylamine (S-NEA) was utilized as chiral derivatization reagent and pre-column derivatization RP-HPLC method was established for...

Journal: :Zeitschrift fur Naturforschung. C, Journal of biosciences 1988
M Wiegand K Eisele

The androgen receptor of murine preputial gland showed in binding experiments a biphasic saturation curve and a biphasic Scatchard plot. The receptor converted from an 8.5-9 S form to a 4.5-5 S form in high ionic strength buffer. The apparent dissociation constant was KD 0.5 +/- 0.2 nM for the 8.5-9 S receptor form. A 6.5-7 S receptor form could be detected in some experiments. The ligand speci...

Journal: :The Biochemical journal 2008
Jung-Yu Tung Margaret Dah-Tsyr Chang Wei-I Chou Yen-Yi Liu Yi-Hung Yeh Fan-Yu Chang Shu-Chuan Lin Zhen-Liang Qiu Yuh-Ju Sun

GA (glucoamylase) hydrolyses starch and polysaccharides to beta-D-glucose. RoGA (Rhizopus oryzae GA) consists of two functional domains, an N-terminal SBD (starch-binding domain) and a C-terminal catalytic domain, which are connected by an O-glycosylated linker. In the present study, the crystal structures of the SBD from RoGA (RoGACBM21) and the complexes with beta-cyclodextrin (SBD-betaCD) an...

Journal: :The Journal of biological chemistry 1988
S W Lowe W J Checovich J Rapacz A D Attie

We previously identified a defect in the in vivo catabolism of low density lipoprotein (LDL) from hypercholesterolemic pigs carrying a mutant apolipoprotein B allele. In the present studies, we examined the in vitro metabolism of mutant LDL in cultured pig fibroblasts. A 3-fold higher concentration of mutant LDL (compared to control) was needed to displace 50% of control 125I-LDL binding. Mutan...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1986
K C Zoon Y Karasaki D L zur Nedden R Q Hu H Arnheiter

Treatment of Madin-Darby bovine kidney (MDBK) cells with human interferon (IFN)-alpha 2 at 37 degrees C results in a dose-dependent inhibition of cell growth and a reduction of the subsequent binding of 125I-labeled epidermal growth factor (EGF) at 4 degrees C. Human IFN-beta and -gamma, which exhibit little antiviral and antiproliferative activities on MDBK cells, have little effect on cell gr...

Journal: :Cancer research 1992
H P Emonard A G Remacle A C Noël J A Grimaud W G Stetler-Stevenson J M Foidart

We have studied the capacity of two human breast adenocarcinoma cells, MDA-MB231 and MCF-7, to bind exogenous M(r) 72,000 type IV collagenase by both morphological and radioreceptor binding assays. By indirect immunofluorescence, staining with a specific anti-M(r) 72,000 type IV collagenase antibody was strongly induced when cells were preincubated with the purified enzyme. Scatchard plot analy...

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