The trichothecene mycotoxin deoxynivalenol (DON), a frequent contaminant of cereal grains, is known to dysregulate mucosal and systemic immunity. In this study, we tested the hypothesis that DON interferes with the murine immune response to viral respiratory infection. Female Balb/c mice (5 weeks old) were orally gavaged with DON (10 mg/kg body weight [bw]) or saline vehicle and then intranasal...

VERWOERD, D. W. & HUISMANS, H . On the relationship between bluetongue, Afr ican horsesickness and reo viruses : hybridization studies. Onderstepoort J. vet. Res., 36 (2) , 175-180, 1969. The double-stranded ribonucleic acid from bluetongue virus (BTV) , African horsesickness virus (AHSV) and reovirus has been tested for hybridization with messe~ger RNA derived from BTV and reovirus-infected ce...

The mechanisms and consequences of persistence of non-transforming viruses are poorly understood. Reovirus infections are usually regarded as cytocidal and infection is associated with inhibition of cellular protein and DNA synthesis. Reovirus infection of the polarized epithelial MDCK cell line is not associated with inhibition of protein synthesis, and cells become persistently infected and c...

Normal and simian virus 40-transformed WI-38 cells exhibited a differential sensitivity to infection with type 3 reovirus. A progressive decrease in viability began 24 to 36 h after infection of transformed cells terminating in complete lysis of cultures by 96 h. Normal cells were productively infected and continued to produce and release virus for as long as 14 days after infection, but exhibi...

Reovirus infections are actually related to a lot of disease conditions with different clinical manifestations. Reoviruses have been isolated from a variety of tissues in poultry, suffering from different disease conditions including viral arthritis/tenosynovitis, stunting syndrome, respiratory disease, enteric disease, immunosuppression and malabsorption syndrome. Economic losses related to re...

Viral infections have been proposed to elicit pathological processes leading to the initiation of T helper 1 (TH1) immunity against dietary gluten and celiac disease (CeD). To test this hypothesis and gain insights into mechanisms underlying virus-induced loss of tolerance to dietary antigens, we developed a viral infection model that makes use of two reovirus strains that infect the intestine ...

For many encephalitis cases, the cause remains unidentified. After 2 children (from the same family) received a diagnosis of acute necrotizing encephalopathy at Centre Hospitalier Universitaire (Tours, France), we attempted to identify the etiologic agent. Because clinical samples from the 2 patients were negative for all pathogens tested, urine and throat swab specimens were added to epithelia...

The agent in wastewater sludge previously shown to reduce the heat required to inactivate reovirus (R. L. Ward and C. S. Ashley, Appl. Environ. Microbiol. 34:681--688, 1977) was "separated" from other sludge components and analyzed by infrared spectroscopy. The infrared spectrum of this material was quite similar to the spectra of commercial anionic detergents, and subsequent analyses of the fr...

Altman, Jennie, "Potent mutagens have positive and negative effects on viral fitness of Reovirus in vitro" (2013). Honors Theses. Paper 22. Due to the inherently error-prone nature of RNA replication, mutations to genomes of RNA viruses occur frequently and accumulate. We hypothesized that RNA versions of nucleoside analogues that increase mutation rates in DNA could cause increases in the muta...

NaClO(4) was employed in a technique for the rapid extraction of reovirus ribonucleic acid (RNA). The extracted RNA, which was purified in a Cs(2)SO(4) equilibrium density gradient, had a buoyant density of 1.61 g/cm(3) and a sedimentation coefficient of 15S in a 7 to 20% sucrose gradient. It was 90% resistant to ribonuclease in a solution of high ionic strength (0.1 m NaCl). The sensitivity of...