DESPITE THE NUMEROUS procedures available for the determination of uric acid, their clinical use remains subject to considerable error. A simple precise method for routine clinical use is desirable since determination of the level of serum uric acid is an important criterion for the diagnosis of gout. Excellent critical reviews of the available methods have been published (1, 2, 3). The current...

Livers from chickens, rats, mongrel dogs, Dalmatian dogs, and man have been examined in the electron microscope in order to compare the microbodies with the known content of uricase. It is concluded that microbodies with inclusion are present in rats, mongrel dogs, and, although the inclusion generally is smaller, in Dalmatian dogs. The inclusion has a characteristic structural appearance. Thes...

Uric acid as measured in serum by three different uricase (EC 1.7.3.3) methods (aca, Ektachem, and SMAC) and by the SMAC method with phosphotungstic acid was compared with a candidate Reference Method for uric acid. Serum specimens from 83 patients (uric acid concentrations, 19 to 141 mg/L) were analyzed by all five methods. Results were compared by using linear regression analysis, and the mea...

A 62-year-old woman receiving chemotherapy with etoposide showed discrepant uric acid values as measured by a direct phosphotungstic acid (PTA) method (150 mg/L) compared with a uricase technique (40 mg/L). After ultrafiltration, the positive interference for the direct PTA method was retained in the protein fraction, but not in the filtrate. Adding exogenous etoposide to drug-free serum confir...

The available methods of determination of uric acid can be divided into three main groups: (a) the direct determination of the reducing power of uric acid; (b) the coprecipitation of the uric acid or its adsorption on a precipitating material, dissolving the acid, and determination of its reducing power; and (c) quantitative decomposition of uric acid by uricase. A survey of these methods has b...

Because the human body cannot synthesize urate oxidase by itself, reducing the level of uric acid requires long-term or even lifelong treatment. However, currently various uric acid-lowering drugs cause different degrees of damage to the human body and thus are not suitable for long-term treatment. A probiotic that can yield a large amount of urate oxidase and be transplanted into the intestine...

Abstract Rasburicase is an expensive treatment used to control hyperuricemia caused by tumour lysis syndrome (TLS). In this study, a non-chromatographic method was designed based on nano-oil bodies for convenient and economical purification of the recombinant uricase. For purpose, two chimaeras were synthesized with different arrangement uricase, caleosin intein fragments. After confirming prot...

Urate oxidase (Uox) catalyses the oxidation of urate to allantoin and is used to reduce toxic urate accumulation during chemotherapy. X-ray structures of Uox with various inhibitors have been determined and yet the detailed catalytic mechanism remains unclear. Neutron crystallography can provide complementary information to that from X-ray studies and allows direct determination of the protonat...

A direct ultraviolet absorption method that does not require uricase for the determination of serum urate is presented. Serum (0.3 ml.) is placed in the bottom of a 25 X 95-mm. shell vial and coagulated by immersion of the vial in boiling water for 2 mm. The clot is overlaid with 3.0 ml. of water and the serum urate extracted for 60 mm. at 37#{176}. The absorbance of the extract is measured at ...