JCB • VOLUME 169 • NUMBER 1 • 2005 16 Folding in a crowd roteins fold better in a crowd, as predicted by a biophysical model from Margaret Cheung, Dmitri Klimov, and D. Thirumalai (University of Maryland, College Park, MD). Interactions between a protein and large nearby molecules produce repulsive forces at distances that can be as large as the protein itself. The group modeled the energetic e...

Phosphorylation is a reversible post-translational modification that regulates many proteins and enzymes, including proteases, as shown by two recent publications. Huang and colleagues and Velázquez-Delgado and Hardy (this issue of Structure) describe how phosphorylation activates the protease activity of the deubiquitinating enzyme DUBA and how it inhibits caspase-6, respectively.

The molecular regulation of skeletal muscle proteolysis and the pharmacological screening of anticatabolic drugs have been addressed by measuring tyrosine release from prepubertal rat skeletal muscles, which are thin enough to allow adequate in vitro diffusion of oxygen and substrates. However, the use of muscle at accelerated prepubertal growth has limited the analysis of adult muscle proteoly...

Wang W., Li Z., Liu J.-Z., Wang Y.-J., Liu S.-H., Sun M. (2013): Comparison between thermal hydrolysis and enzymatic proteolysis processes for the preparation of tilapia skin collagen hydrolysates. Czech J. Food Sci., 31: 1–4 The tilapia (Oreochromis niloticus) skin hydrolysate was produced by thermal or enzymatic hydrolysis processes. Several product characteristics were studied such as the av...

To shed further light onto the mechanisms of proteolysis that prevail throughout ripening of Portuguese cheeses, model cheeses were manufactured from bovine milk, following as much as possible traditional manufacture practices - using either animal or plant rennet. The individual role upon proteolysis of two (wild) strains of lactic acid bacteria - viz. Lactococcus lactis and Lactobacillus brev...

All cells contain proteases, which effect catalytic hydrolysis of the peptide bond between amino acids in the protein backbone. Typically, proteinases are prevented from nonspecific proteolysis by regulation and physical separation into different subcellular compartments; however, this segregation is not retained during cell lysis to release a protein of interest. Prevention of proteolysis duri...