dna amplification using taq dna polymerase is one of the most widely used techniques in molecular biology and biotechnology. the aim of this study was to amplify the gene of this enzyme from a thermophilic bacteria called thermus aqauticus and clone it into a vector for future use. using specific primers the cdna of taq dna polymerase was amplified and ligated into the cloning vector ptz57r usi...

the last two decades have seen a massive growth in the application of dna technology in histocompatibility and immunogenetics. this review summarises the history and application of dna methods in this area.

conclusions performing only culture method led to missed false negative carriers. therefore, it is recommended that both the pcr assay and conventional culture method be routinely performed to detect gbs in pregnant women accurately. pcr diagnosis demonstrated a shorter turnaround time when compared with the time consuming culture method. results the prevalence of gbs in the 203 collected sampl...

conclusions torghabeh - shandiz district with a rural texture is endemic for anthroponotic cutaneous leishmaniasis (acl). further studies are recommended to discover the vectors and probable reservoirs in these areas. results of 164 samples obtained from the skin lesions of the patients referred to the torghabe - shandiz health centers, leishmania dna was isolated from 136 smears by pcr method....

conclusions evidence of t. gondii occurrence in sheep and camels was provided in the provinces under study. there is a need to investigate the potential risk factors of zoonotic infections. furthermore, animal health and production losses caused by the parasite; and associated zoonotic implications in the area under study need to be explored. materials and methods animals in the field and those...

background cholera is a severe diarrheal illness caused by vibrio cholerae, which produces a virulence factor named el tor hemolysin encoded by the hlya gene. objectives this study meant to analyze the phenotypic characteristics and presence of hlya gene in v. cholerae isolated from patients in iran. the hlya gene which codes for hemolysin, plays an essential role in manifestation of cholera ,a...

background brucellosis is the most common global zoonosis and an important public health problem in many parts of the world including iran. diagnosis of brucellosis is frequently difficult to establish and conventional methods are not always successful in identifying the organisms. rapid detection of brucella species by an automated blood culture system and polymerase chain reaction (pcr) may l...

background: some dynamic changes occurs during spermatogenesis such as histone removal and its replacement with transition nuclear protein and protamine. these proteins are required for packing and condensation of sperm chromatin. jhdm2a is a histone demethylase that directly binds to promoter regions of tnp1 and prm1 genes and controls their expression by removing h3k9 at their promoters.objec...

DNA misalignment occurs in homopolymer tracts during replication and can lead to frameshift mutations. Polymerase (pol) recognition of primer-templates containing bulge structures and the transmission of a bulge through a polymerase binding site or replication complex are important components of frameshift mutagenesis. In this report, we describe the interaction of the catalytic core of pol wit...

Termination of transcription by RNA polymerase II requires two distinct processes: The formation of a defined 3' end of the transcribed RNA, as well as the disengagement of RNA polymerase from its DNA template. Both processes are intimately connected and equally pivotal in the process of functional messenger RNA production. However, research in recent years has elaborated how both processes can...