I. The two isozymes of alkaline phosphatase (orthophosphoric monoester phosphohydrolase, EC 3.1.3.1) detectable in human blood group O plasma have been separated from one another and isolated in a high state of purity. With p-nitrophenyl phosphate as substrate, the catalytic properties of the two isozymes appear to be the same with respect to pH optimum, lO.5; Kin, o.35-o.4 mM, and activity in ...

Fast protein liquid chromatography (anion exchange) was used to separate glutathione S-transferase isozymes in nontreated etiolated maize shoots and those treated with the herbi­ cide safener CGA-154281 4-(dichloroacetyl)-3,4-dihydro-3-methyl-2 H-l ,4-benzoxazine. Non­ treated shoots contained isozymes active with the following substrates: fra/?s-cinnamic acid (1 isozyme), atrazine (3 isozymes)...

Two isozymes of 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase are partitioned into plastid (DS-Mn) and cytosolic (DS-Co) compartments of at least several higher plants (RA Jensen 1986 Rec Adv Phytochem 20: 257-258). Differential variation of isozyme levels and in the timing of their expression was observed during growth of Nicotiana silvestris in suspension culture. The ratio of DS-Co to...

Chromatographic separation of the non-heme proteins from the erythrocytes of the subterranean mole rat belonging to the superspecies Spalax ehrenbergi from Israel revealed two major peaks. On sequence analyses, the larger peak corresponded to a 56 kDa selenium-binding protein (SeBP) previously characterized from mouse and human liver, and the second peak to the low-activity carbonic anhydrase (...

A two site enzyme immunoassay which quantitatively identifies types I, II, and III of protein kinase C isozymes has been designed. The soluble protein kinase C isozymes were selectively immobilized by type-specific monoclonal antibodies, MC-1a, -2a, and -3a (H. Hidaka et al., J Biol. Chem., 263: 4523-4526, 1988) which bind to the regulatory domain (NH2-terminal side) of protein kinase C. The am...

Heme oxygenase (HO) is the rate-limiting enzyme in the degradation of heme to produce bile pigments and carbon monoxide. The HO-1 isozyme is induced by a variety of factors such as heat, heme, ischemia, and hydrogen peroxide. In recent years, mounting findings have suggested that HO-1 has a neuroprotective activity against ischemic injury. The neuroprotective role of isoflurane, a commonly used...

conclusions the bleaching procedures using 20% carbamide peroxide and 45% carbamide peroxide and diode laser significantly decreased shear bond strength of brackets to the teeth. 45% carbamide peroxide had a more significant effect on bond strength compared to 20% carbamide peroxide. the difference in bond strength was not significant between laser group and either carbamide peroxide groups. re...

Electrophoresis is currently the best technique for quantifying inherited variation which has contributed to our knowledge of population biology in revealing population structure. Considerable variation in isozymes of diamondback moth, Plutella xylostella (L.), was detected. Esterase zymograms were prepared from 3rd and 4th instar larvae, pupae and adults. There were two groups of esterase band...

Intracellular glutathione (GSH) levels for seven mammalian cell lines (four human tumors, two rodent, one monkey) were determined by flow cytometry following staining with monochlorobimane (MBCl), and the results were compared with GSH levels measured by the Tietze assay. The mean fluorescence intensity for all but the two rodent lines did not correlate with GSH levels determined biochemically....