Objective The French Polynesia Zika virus (ZIKV) outbreak (2013–2014) broadened the known manifestations of ZIKV disease (ZVD) to include neural and congenital syndromes. A subsequent epidemic in Americas (2015–2016) caused >685,000 suspected/confirmed cases before seemingly disappearing as fast it expanded. study was implemented (2017–2018) detect ZVD region (Mexico, Honduras, Colombia ...

Aims-To develop a rapid PCR ELISA procedure for the detection of mutations in K-ras in a microtitre plate format, and to evaluate the assay for the detection of these mutations in human colorectal cancer.Methods-An enriched PCR method was used with labelled primers, and PCR product was captured on GCN4 coated immunoassay plates. Detection of biotinylated mutant product was performed by colorime...

Background/aim: This study included patients who had digestive system complaints between August 2015 and October 2015. The research was designed to compare conventional microscopy with an antigen detection ELISA kit and the TaqMan-based real-time PCR (RT-PCR) technique for detection of Giardia intestinalis in human stool specimens. Materials and methods: Samples were concentrated by formalin-et...

The performance of different in vitro diagnostic tests for the diagnosis of invasive aspergillosis (IA) was investigated in a transiently neutropenic rat model. Rats were immunosuppressed with cyclophosphamide and then inoculated intravenously with 1.5 x 10(4) CFU Aspergillus fumigatus spores. Animals were then either treated with caspofungin acetate, 1 mg/kg/day for 7 days, or not treated. PCR...

Until recently, sheep-associated malignant catarrhal fever (SA-MCF) was diagnosed mainly on the basis of clinical presentation and histopathological changes. Using clinically diagnosed field cases, we have evaluated a seminested PCR and a competitive inhibition enzyme-linked immunosorbent assay (CI-ELISA) and compared these assays in the diagnosis of SA-MCF in cattle with histopathology as a pr...

BACKGROUND Hepatitis E virus (HEV) genotype 3 infections are frequent in Europe and North America, with acute and chronic courses described in the literature. HEV RNA detection by real-time polymerase chain reaction (PCR) is the gold standard for diagnosis. Recently, an anti-HEV antigen (Ag)-specific enzyme-linked immunosorbent assay (ELISA) directed against the HEV capsid became commercially a...

background and aims: infectious mononucleosis (im)is the clinical manifestation of primary infection with epstein-barr virus (ebv). humans are the only known reservoir of ebv. regarding the problems in diagnosis of the disease, the purpose of this study was to assess enzyme-linked immunosorbent assay (elisa) and nested polymerase chain reaction (pcr) as a diagnostic tool for this disease. mater...

This study aimed to investigate the phenolic content, antioxidant activity, cytotoxicity and in vitro wound healing activity of methanolic aqueous extracts Verbascum stachydifolium Boiss & Heldr. var. stachydifolium. Total flavonoid contents were measured using spectrophotometry-based methods. Quantitative analysis selected phenolics was performed by HPLC. The cytotoxic effects on L929 mous...

Monitoring of poultry, including domestic ducks, for avian influenza (AI) virus has increased considerably in recent years. However, the current methods validated for the diagnosis and detection of AI virus infection in chickens and turkeys have not been evaluated for performance with samples collected from domestic ducks. In order to ensure that methods for the detection of AI virus or AI viru...

Methods: Sow herds were screened for antibodies to PCV2 by ELISA, with sample-to-positive (S:P) ratios ≥ 0.2 considered positive. Piglets were obtained by segregated early weaning (SEW) at 10 to 12 days of age. Maternal anti-PCV2 antibody was determined by testing weekly blood samples by ELISA and indirect immunofluorescence assay. Absence of PCV2 was determined by polymerase chain reaction (PC...