A PCR method suitable for the isolation of lipase genes directly from environmental DNA is described. The problems associated with the low levels of similarity between lipase genes were overcome by extensive analysis of conserved regions and careful primer design. Using this method, a lipase gene (oli-lipase) was isolated directly from environmental DNA. This lipase showed less than 20% similar...

To oli showed that every cellular automaton of an arbitrary dimension d can be embedded into a reversible cellular automaton of dimension d+1. He asked \whether an arbitrary cellular automaton can be embedded in a reversible one having the same number of dimensions" and conjectured that this is not possible. We show that his conjecture is true. Even if one imposes only a weak, natural condition...

Michel e C eccarelli Isti t uto di Ri cerca sui Sist emi In form atici Perelleli, IR SIP-CNR 80125 Nap oli, It aly Ab stract. Additive automata are analyzed and used as associative memories. Th eir storage capacity is comput ed as well as the attrac tion basin of each memorized pattern. We show how such systems can be used when the patterns to be memorized have a subset of relevant features; "s...

The nucleotide sequence of mitochondrial DNA of a cytoplasmic "petite" mutant of Saccharomyces cerevisiae is reported. The DNA has a repeat length of 1060 base pairs and contains a genetic marker (oli-1) for the ATPase proteolipid. The nucleotide sequence reveals the presence of part of the structural gene of the subunit-9 proteolipid of the ATPase complex and an extended A+T-rich region adjace...