A successful protocol was established for micropropagation in two selected varieties of exotic ornamental plants, Calathea crotalifera. The effects of different sterilization techniques, explant type, and the combination and concentration of plant growth regulators on shoots induction were studied. The axillary shoot buds explants sprouted from rhizomes in soil free conditions showed high induc...

Atalantia monophylla (L.) DC. is a medium sized medicinal tree used in Ayurveda, Siddha and Traditional systems of medicine. It is used for the treatment of chronic rheumatism and paralysis, as an antidote for snake bites and shows antibacterial, antifungal and antifeedant activity. The study aims to develop a protocol for multiple shoot induction from nodal and root explants. After surface ste...

Shoot meristems used for the study were exercised from the in vitro regenerated shoots cultured on MS medium supplemented with 0.5 mg/L of BAP for multiplication. The sensitivity of the in vitro regenerated was studied using shoot meristems of 0.5 cm. Shoot meristems were cultured on medium containing 10 100 mg/l kanamycin to determine the concentration that was lethal for multiple shoot induct...

A complete protocol is presented for in vitro regeneration of pomegranate (Punica granatum L.), a tropical fruit tree, using cotyledonary nodes derived from axenic seedlings. Shoot development was induced from cotyledonary nodes on Murashige and Skoog (1962) (MS) medium supplemented with 2.3±23.0 mM benzyladenine (BA) or kinetin (Kn). Both type and concentration of cytokinin signi®cantly in ̄uen...

Wounding of plants leads to endogenous rise of jasmonic acid (JA) accompanied with the expression of a distinct set of genes. Among them are those coding for the allene oxide cyclase (AOC) that catalyses a regulatory step in JA biosynthesis, and for 1-deoxy-D-xylulose 5-phosphate synthase 2 (DXS2), an enzyme involved in isoprenoid biosynthesis. To address the question how roots and shoots of Me...

A survey was conducted over 12 months to determine productivity and compare the relationships between the different shoot types of three rose cultivars of different ages. The plants were trained in a `vase-shaped' architecture. Data were collected on 1 year-old `Sonia', 8 year-old `Gabrielle' and 7 year-old `Kardinal'. The ratio of basal shoots : branches : shoots ( ̄owering and non̄owering shoot...

In this research, a factorial 4×3×4 with 4 replications in Completely Randomized design was used to study the effects of different auxin treatments (4 levels), pH(2 levels) and cultivars (3 levels) on regeneration and rooting of in vitro shoot tips excised from shoots cultured on media supplemented with 1.5-2 mg/l BA. The results showed both auxins (NAA and IBA) produced callus in the bases of ...

It is well known that a long defoliated piece of stem of Bryophyllum calycinum forms shoots only at the apical or the two apical nodes, while when such a stem is cut into as many pieces as there are nodes each node produces shoots. It is shown in this paper that the dry weight of shoots produced in the apical nodes of a long piece of stem is approximately equal to the dry weight of shoots the s...

With the aim of micropropagation of Phyllanthus amarus, an important medicinal herb, shoot tips were cultured in Murashige and Skoog's medium supplemented with kinetin/ BAP singly or in combination with IAA. Growth regulators at lower range (0.1-1.0 mg L(-1)) stimulated direct regeneration of shoots. Kinetin was superior to BAP and kinetin-IAA combination was more suitable than kinetin alone. A...

A rapid and efficient protocol for the large-scale propagation of an important woody medicinal plant, Cassia alata L., is described. Cotyledonary node (CN) obtained from 15 day-old aseptic seedling were used as an explant and their morphogenetic potential was tested on MS media with various concentrations (0.5–12.5 μM) of 6-benzyladenine (BA), Kinetin (Kn), and 2-isopentinyl adenine (2iP) alone...