Extracts of Hypericum perforatum (St. John's Wort) are widely used for the treatment of depressive disorders and are unspecific inhibitors of the neuronal uptake of several neurotransmitters. Previous studies have shown that hyperforin represents the reuptake inhibiting constituent. To characterize the mechanism of serotonin reuptake inhibition, kinetic analyses in synaptosomes of mouse brain w...

Studies were conducted to determine the effects of lysocellin and monensin on mineral metabolism of steers fed forage-based diets. In each study treatments consisted of 1) control, 2) 100 mg lysocellin/d, 3) 200 mg lysocellin/d and 4) 200 mg monensin/d. Twenty-four growing Hereford steers were used in each of two experiments to evaluate the effects of ionophore feeding on plasma and ruminal sol...

A dual-flow continuous culture system was used to evaluate the effects of laidlomycin propionate and bacitracin zinc on fermentation, nutrient digestibility, microbial efficiency. Factors were (LP; 2 mg/L volume) (BAC; 1.4 mg/L), treatments as follows: (1) no BAC or LP (CON), (2) without (LP), (3) (BAC), (4) (LP/BAC). fifth treatment supplemented with monensin sodium (MON; 6 mg/L) act a positiv...

The Na+ ionophore monensin affects cellular pH and, depending on its concentration, causes the survival or death of tumor cells. In the present study, we elucidated the survival pathway activated in U937 cells, a human lymphoma-derived cell line. These cells treated with monensin at a concentration of 5 µM were growth-arrested in G1, activated p38 mitogen-activated protein kinase (MAPK) and sho...

A 2-yr study was conducted during the 2004 to 2005 (YR1) and 2005 to 2006 (YR2) winter wheat grazing seasons to determine the effects of supplementation strategies and delivery methods on supplement intake and growth performance of grazing steers (YR1, n = 253, initial BW 255 +/- 25 kg; YR2, n = 116, initial BW 287 +/- 14 kg). The 5 treatments were as follows: 1) negative control (NC), no suppl...

The monovalent ionophore monensin inhibits the secretion of both procollagen and fibronectin from human fibroblasts in culture . The distribution of these proteins in control and inhibited (5 x 10-7 M monensin) cells has been studied by immunofluorescence microscopy . In control cells, both antigens are present throughout the cytoplasm and in specific deposits in a region adjacent to the nucleu...

the efficacy of clindamycin and monensin were evaluated in the prevention of oocyst shedding of kittens with toxoplasma gondii (tehran strain). in this study, 28 healthy kittens aged 1.5 – 2 months old divided randomly into 4 groups of seven. in group 1, kittens were fed with the infected brain tissues of mice, all of seven kittens (100 %), shed oocyst, nearly 1 week after infection, which last...

alkaline complexes by X-ray crystallography,8) Seyeral studies on the chemical modification of the monensin skeleton have been reported, either on the Ci-Cs carboxylic moiety,9・iO) or at the C,,-hydroxyl group,ii) fo11owing the isolation of antibiotics X-14667A and Bi2) (monensin C26-phenethyl urethans). We have shown that the C26-chloro-4 phenyl urethan derivative had a transport selectivity, ...

A series of in vitro and in vivo trials was conducted to determine if continuous monensin feeding for up to 56 d would reduce ruminal conversion of L-tryptophan (TRP) to 3-methylindole (3MI). Fourteen mature beef cows were adapted to a maintenance diet for 3 wk. In trial I, the sampling time to optimize 3MI production was determined. Trials II through IV were to determine the duration of effica...