Oxytetracycline (OTC) significantly inhibited cytochrome c oxidase activity in bovine lymphocytes infected with Theileria parva and in uninfected mitogen-stimulated lymphocytes. The inhibitory effect was detected in vitro within 24 h of treatment with drug concentrations as low as 1 micrograms/ml. Following mitogen stimulation of lymphocytes, concentrations of 3 and 10 micrograms/ml OTC complet...

Mitogen blastogenic responses of lymphocytes from dogs infested with adult Rhipicephalus sanguineus and the effects of salivary gland extracts (SGE) of the tick on the blastogenic responses of lymphocytes from normal dogs were studied. Infestation by R. sanguineus significantly suppressed concanavalin A, phyto-hemagglutinin and pokeweed mitogen responses of lymphocytes from dogs. The inhibition...

Hydrogen peroxide may serve as a diffusible intermediary messenger under physiological and pathophysiological conditions. H2O2 (1 mM) increased the amplitude of GABAergic miniature inhibitory postsynaptic currents in substantia gelatinosa of the spinal cord. This effect of H2O2 was reversed by the p38 antagonist SB203580, but not by the extracellular signal-regulated kinase 1/2 antagonist PD980...

Proliferating myoblasts express the muscle determination factor, MyoD, throughout the cell cycle in the absence of differentiation. Here we show that a mitogen-sensitive mechanism, involving the direct interaction between MyoD and cdk4, restricts myoblast differentiation to cells that have entered into the G0 phase of the cell cycle under mitogen withdrawal. Interaction between MyoD and cdk4 di...