conclusions this study showed that multiple β-lactamases can be produced in burn isolates. this suggests that use of cephalosporins and carbapenems should be restricted in burn isolates to minimize the development and spread of these multidrug resistant pathogens. results there was 99% resistance to carbenicillin, and ticarcillin, 98% to cotrimoxazole, 96% to ciprofloxacin, and aztreonam, 95% t...

Pseudomonas aeruginosa COL-1 was identified in a blood culture of a 39-year-old-woman treated with imipenem in Marseilles, France, in 1996. This strain was resistant to beta-lactams, including ureidopenicillins, ticarcillin-clavulanic acid, cefepime, ceftazidime, imipenem, and meropenem, but remained susceptible to the monobactam aztreonam. The carbapenem-hydrolyzing beta-lactamase gene of P. a...

Aim: In this study, we evaluated the presence of antibiotic resistance genes among P. aeruginosa strains. Methodology: From January to September 2012, 100 isolates of P. aeruginosa were collected from burn patients. Antimicrobial susceptibility testing was performed by disk diffusion method. Screening for Metallo-β-lactamases (MBLs) productions were performed by Combination Disk Diffusion Test ...

Bacterial sepsis is one of the major causes of morbidity and mortality among neonates. It is characterized as generalized bacterial infection indicated by positive blood culture in the first four weeks of life (Agarwal et al., 2001). The common causative agents for neonatal sepsis are Staphylococcus spp, Enterococci spp, E. coli, Klebsiella spp, Enterobacter spp, Pseudomonas aeruginosa and Acin...

New Delhi metallo-beta-lactamase-1 (NDM-1) is a bacterial enzyme that renders the bacteria resistant to a variety of beta-lactam antibiotics. A 20-year-old man was hospitalized several times for surgical treatment and complications caused by a right-sided vestibular schwannoma. Although the patient acquired several multidrug-resistant infections, this study focuses on the NDM-1-producing Acinet...

Infections due to carbapenem-resistant Klebsiella pneumoniae represent a growing problem nationally. In our study, we aimed to examine carbapenem-resistant K. pneumoniae with multiple resistance isolated in the intensive care unit of our hospital. Isolates were investigated for the presence of oxacillinase and metallo-beta lactamase genes with a view to determining the clonal relationship betwe...

BACKGROUND Pseudomonas aeruginosa is an important nosocomial pathogen, commonly causing infections in immunocompromised patients. The aim of this study was to examine the genetic relatedness of metallo-beta-lactamase (MBL) producing carbapenem resistant Pseudomonas aeruginosa clinical isolates collected from 2 tertiary hospitals in Cairo, Egypt using Multi Locus sequence typing (MLST). METHOD...

Generation of the polyadenylated 3' end of an mRNA requires an endonucleolytic cleavage followed by synthesis of the poly(A) tail. Despite the seeming simplicity of the reaction, more than a dozen polypeptides are required, and nearly all appear to be necessary for the cleavage reaction. Because of this complexity, the identity of the endonuclease has remained a mystery. Here we present evidenc...

BACKGROUND & OBJECTIVES Carbapenemase-producing Enterobacteriaceae isolates have been increasingly identified worldwide. Though molecular data regarding New Delhi metallo-beta-lactamase-1 (NDM-1) producers are available, data regarding their rate of infection in a hospital setting and percentage among different clinical isolates are scarce. Hence, this study was undertaken to determine the occu...

BACKGROUND Metallo-β-lactamases are bacterial enzymes that provide resistance to carbapenems, the most potent class of antibiotics. These enzymes are commonly encoded on mobile genetic elements, which, together with their broad substrate spectrum and lack of clinically useful inhibitors, make them a particularly problematic class of antibiotic resistance determinants. We hypothesized that there...