MPSS (massively parallel signature sequencing) is a sequencing-based technology that uses a unique method to quantify gene expression level, generating millions of short sequence tags per library. We have created a series of databases for four species (Arabidopsis, rice, grape and Magnaporthe grisea, the rice blast fungus). Our MPSS databases measure the expression level of most genes under def...

In prokaryotes and eukaryotes, multidrug resistance (MDR) transporters use energy-dependent efflux action to regulate the intracellular levels of antibiotic or xenobiotic compounds. Using mutational analysis of ABC3, we define an important role for such MDR-based efflux during the host penetration step of Magnaporthe grisea pathogenesis. Mutants lacking ABC3 were completely nonpathogenic but we...

We solated and determined the complete nucleotide sequence of a ras gene from Botryotinia fuckeliana, the causal agent of gray mold disease. Furthermore, in two other phytopathogenic fungi, Magnaporthe grisea (rice blast fungus) and Colletotrichum lagenarium (an anthracnose fungus), ras gene homologs were identified by amplifying gene fragments in polymerase chain reactions. The putative ras ge...

Alternative splicing creates a diversity of gene products in higher eukaryotes. Twenty-five percent (1,583/ 6,371) of predicted alternatively spliced transcripts can be detected using the NSF45K rice whole-genome oligonucleotide array. We used the NSF45K array to assess differential expression patterns of 507 loci showing at least a twofold change in expression between lightand darkgrown seedli...