Somatic embryogenesis has been accomplished from tender leaf base explant of date palm (Phoenix dactylifera L.). Three to five mm long tender leaf base explants derived from the meristamatic region of 2-3 year old offshoots of date palm cv. Kheneizi were cultured on Murashige and Skoog (MS) basal medium supplemented with 10, 50, 100 and 150 mg l 2,4-dichlorophenoxy acetic acid (2,4-D) and incub...

The DNA methylase inhibitors, 5-azacytidine and 5-aza-2'-deoxycytidine inhibited adventitious shoot induction in Petunia leaf cultures. Cytosine methylation status at CCGG sites in shoot- and callus-inducing culture treatments was analysed by coupled restriction enzyme digestion (HpaII or MspI) and random amplification. Two differentially methylated genomic DNA bands from the PCR products were ...

Poplar is a model organism for high in vitro regeneration in woody plants. We have chosen a hybrid poplar Populus davidiana Dode x Populus bollena Lauche. By optimizing the Murashige and Skoog medium with (0.3 mg/L) 6-benzylaminopurine and (0.08 mg/L) naphthaleneacetic acid, we have achieved the highest frequency (90%) for shoot regeneration from poplar leaves. It was also important to improve ...

This study describes a Platycerium bifurcatum (Cav.) C. Chr. propagation method through initiation of green globular bodies (GGBs) from juvenile leaf explants of in vitro grown sporophytes. The GGB initiation was first obtained by incubating the explants on MS medium supplemented with α-naphthalene acetic acid (NAA) and two other cytokinins, 2-isopentenyl adenine (2ip) or 6-benzylaminopruine (B...

In the present study, phytochemical constituents and in vitro antioxidative potential of different explants of E. aureum were evaluated. Methanol was used as solvent for extraction of E. aureum three explants (leaf, root and stem). Phytochemical screening was done by performing different chemical tests and presence of carbohydrate, protein, steroid, glycosides, alkaloids, saponins and amino aci...

Vitex trifolia is a shrub species with popular use as a medicinal plant, for which leaves, roots and flowers have been reported to heal different distresses. The increasing exploitation of these plants has endangered its conservation, and has importantly justified the use of biotechnological tools for their propagation. Our aim was to present an efficient protocol for plant regeneration through...

in vitro propagation of rose (rosa indica) as well as rose breeding is a valuable economic enterprise and has received highest importance globally including indian subcontinent. the development of new cultivars for commerce is faced with new challenges. in vitro micropropagation protocols of varieties of roses have been established but very few reports on the clonal propagation of local cultiva...

Ethanol extracts from the different parts of B. orellana showed differential antimicrobial activity. It was found that the extracts of in vitro leaves showed maximum activity against Bacillus pumilus followed by the extracts from the roots and hypocotyls. The callus derived from different explants too showed antimicrobial activity. The leaf callus showed maximum activity. The zone of inhibition...

Background Leaf abscission is an important trait for biomass production and seasonal acclimation in deciduous trees of the temperate region. Various plant hormones are involved in the timing of abscission. For example, ethylene signaling is required to induce hydrolysis of cell walls, while an auxin gradient [1] was suggested to act upstream of ethylene on the onset of leaf abscission. Besides ...

Drought is one of the major limitations to crop production worldwide. This study was conducted to evaluate the response of five almond genotypes and peach×almond hybrid GF to drought stress in vitro, and screening drought tolerance. Explants subjected to polyethylene glycol osmotic stress ( , , and . % WV) on the MS medium. Increasing PEG level in the medium significantly reduced fresh weight a...