Chromosomal double-strand breaks (DSBs) in mammalian cells are repaired by either homology-directed repair (HDR), using a homologous sequence as a repair template, or nonhomologous end-joining (NHEJ), which often involves sequence alterations at the DSB site. To characterize the interrelationship of these two pathways, we analyzed HDR of a DSB in cells deficient for NHEJ components. We find tha...

Nonhomologous end joining (NHEJ) plays a major role in double-strand break DNA repair, which involves a series of steps mediated by multiprotein complexes. A ring-shaped Ku70/Ku80 heterodimer forms first at broken DNA ends, DNA-dependent protein kinase catalytic subunit (DNA-PKcs) binds to mediate synapsis and nucleases process DNA overhangs. DNA ligase IV (LigIV) is recruited as a complex with...

AMA Jakub R, Filip M, Daniel B, et al. Nuclear expression of Ku70/80 is associated with chek2 germline mutations in breast cancer. Polish Journal Pathology. 2023. doi:10.5114/pjp.2023.129518. APA Jakub, R., Filip, M., Daniel, B., Jolanta, H., Tomasz, & Cezary, C. (2023). https://doi.org/10.5114/pjp.2023.129518 Chicago Rosik, Machaj Bodnar Hybiak Huzarski Cybulski and Lubiński Jan "Nuclear cance...

PURPOSE To determine the biological effectiveness of single, fractionated and continuous low dose rate irradiation on the human colorectal cancer cell line CL187 in vitro and explore the cellular mechanisms. MATERIALS AND METHODS The CL187 cells were exposed to radiation of 6 MV X-ray at a high dose rate of 4Gy/min and 125I seed at a low dose rate of 2.77 cGy/h. Three groups were employed: si...