1. The matrix pyrophosphate (PPi) content of isolated energized rat liver mitochondria incubated in the presence of ATP, Mg2+, Pi and respiratory substrate was about 100 pmol/mg of protein. 2. After incubation with sub-micromolar [Ca2+], this was increased by as much as 300%. There was a correlation between the effects of Ca2+ on PPi and on the increase in matrix volume reported previously [Hal...

Transport of GSH was studied in isolated rat kidney cortical brush-border membrane vesicles in which gamma-glutamyltransferase had been inactivated by a specific affinity labeling reagent, L-(alpha S,5S)-alpha-amino-3-chloro-4,5-dihydro-5-isoxazoleacetic acid (AT-125). Transport of intact 2-3H-glycine-labeled GSH occurred into an osmotically active intravesicular space of AT-125-treated membran...

VLA-2 (also called gpla/IIa on platelets) is a collagen receptor with a unique ot subunit and a subunit common to other adhesion receptors in the VLA/integrin family. Multiple cDNA clones for the human VLA-2 o~ 2 subunit have been selected from a hgtll library by specific antibody screening. The 5,374bp nucleotide sequence encoded for 1,181 amino acids, including a signal peptide of 29 amino ac...

Intact or Cys-34 modified human serum albumin (HAS) was significantly damaged and degraded in the metal-catalyzed oxidation (MCO) system containing dithiothreitol (DTT) as an electron donor and, while it lasted, this protein might have prohibited the •OH or H2O2 from attacking DNA. However, in the glutathione (GSH) and ascorbate (non-thiol) MCO system, HSA was not sacrificially degraded and acc...

Suramin is a large naphthyl-polysulfonate compound that inhibits an array of receptors and enzymes, and it has also been reported to block currents mediated by glutamate receptors. This study shows that suramin and several structurally related compounds [8,8 -[carbonylbis(imino-3,1-phenylenecarbonylamino)]bis-(1,3,5naphthalenetrisulfonic acid), 6Na (NF023), 8,8 -(carbonylbis(imino-4,1-phenylene...

IGhi satellite of l h x-ray fluorescence spectra were measured with two-crystal spectrometer. The intensity of the multivacancy satellite depends on the covalency of the orbital from which the electron(s) is(are) shaken off. However if a resonance condition holds, then the satellite is anomalously weak due to resonant electron transfer (RET) from the center atom to the ligands. Some examples of...