aacel9a [β-1,4-endoglucanase, (e.c:3.2.1.4)], was immobilized onto glutaraldehyde activated chitosan macrosphere by covalent attachment. the properties of the immobilized aacel9a were investigated by determining the optimum ph and optimum temperature for activity, thermal stability, and kinetic parameters. the immobilization process shifted the enzyme’s optimum temperature from 65 °c for the fr...

During aggregation the larger Dictyostelium species use cAMP as a chemoattractant and possibly also as a transmitter. In passage from cell to cell, cAMP levels are modulated by diffusion and by enzyme hydrolysis. It appears that the important cAMP-hydrolysing enzyme is a phosphodiesterase bound to the cell membrane, the main roles of which are (1) very fast hydrolysis of cAMP and (2) steepening...

Lecitase Ultra® solutions are mainly composed of bimolecular aggregates two open structures the enzyme. The immobilization and fixation these onto support surfaces is here proposed as a novel protocol for stabilization Lecitase. resulting derivatives were much more stable than diluted most them was obtained by covalent aggregate: 300-fold enzyme 75-fold adsorbed hydrophobic supports. aggregate ...

BACKGROUND We and other workers have shown that accessory enzymes, such as β-glucosidase, xylanase, and cellulase cofactors, such as GH61, can considerably enhance the hydrolysis effectiveness of cellulase cocktails when added to pretreated lignocellulosic substrates. It is generally acknowledged that, among the several factors that hamper our current ability to attain efficient lignocellulosic...

Aalto University, P.O. Box 11000, FI-00076 Aalto www.aalto.fi Author Ville Pihlajaniemi Name of the doctoral dissertation Pretreatment categories, process alternatives and material characteristics in enzymatic hydrolysis of lignocellulose: Publisher School of Chemical Technology Unit Department of Biotechnology and Chemical Technology Series Aalto University publication series DOCTORAL DISSERTA...

Citation: Krupke A, Hmelo LR, Ossolinski JE, Mincer TJ and Van Mooy BAS (2016) Quorum Sensing Plays a Complex Role in Regulating the Enzyme Hydrolysis Activity of Microbes Associated with Sinking Particles in the Ocean. Front. Mar. Sci. 3:55. doi: 10.3389/fmars.2016.00055 Quorum Sensing Plays a Complex Role in Regulating the Enzyme Hydrolysis Activity of Microbes Associated with Sinking Particl...

Cells signal through lipids produced by phospholipid and phosphoinositide metabolism that involves three enzymic processes: (i) ester and phosphodiester hydrolysis by phospholipases; (ii) monophosphate hydrolysis by phosphatases; and (iii) phosphorylation of hydroxy groups by kinases. Unregulated enzyme activity correlates with specific pathologies, which are specific targets for therapeutic in...

The hydrolysis of two cephalosporins by three different beta-lactamases has been studied. Each enzyme caused a decrease in ultraviolet absorption, a loss of biological activity, and the release of the leaving group from the 3-position. The changes occurred at the same rate and to the same extent with each enzyme, and it is inferred that the loss of the leaving group is a consequence of, and not...

Recombinant Chlamydomonas reinhardtii chlorophyllase 1 (CrCLH1) that could catalyze chlorophyll hydrolysis to chlorophyllide and phytol in vitro was successfully expressed in Escherichia coli. The recombinant CrCLH1 was immobilized through covalent binding with a cubic (3-aminopropyl) triethoxysilane (APTES) coating on magnetic iron oxide nanoparticles (MIONPs), which led to markedly improved e...

The existence and biochemical functions of flavin adenine dinucleotide (FAD) have been well recognized in plants as well as in other living cells. There are some informa tions concerning the biosynthesis of FAD from flavin mononucleotide (FMN) and ATP by a reversible reaction, catalzyed by FAD pyrophosphorylase in beans (1), yeast (2, 3) and animals (4, 5, 6). On the other hand, the FAD hydroly...