OBJECTIVE There is increased interest in measuring peripheral oxytocin levels to better understand the role of this peptide in mammalian behavior, physiology, and disease. The purpose of this study was to compare methods for plasma oxytocin measurement using a commercially available enzyme immunoassay (EIA) and radioimmunoassay (RIA), to evaluate the need for sample extraction, and to assess th...

In public health studies limited volumes of blood are often collected and stored for future hypothesis testing. Archived samples are irreplaceable and therefore it is valuable to develop analytical techniques that require minimal sample volume. This work describes the measurement of trace elements Mg, Cu, Fe, Zn and ultratrace elements Cd, Co, Mn, Pb in limited quantity (150 μL) human serum or ...

background: in this study we have prepared the iga solution from normal human plasma using plasma fractionation and ion exchange chromatography. materials and methods: using fractionation of plasma with cold ethanol (starting with 8% ethanol), fraction iii was prepared as a suitable source for iga preparation. then it was treated with caprylic acid for separation of impurities. for enrichment o...

We tested the hypothesis that apolipoproteins, the protein constituents of plasma lipoproteins, are secreted into bile. We examined human gallbladder bile obtained at surgery (N = 54) from subjects with (N = 44) and without (N = 10) gallstones and hepatic bile collected by T-tube drainage (N = 9) after cholecystectomy. Using specific radioimmunoassays for human apolipoproteins A-I and A-II, the...

background: lipid peroxidation is a free radical-generating process which occurs on every membranous structure of the cell. free radicals are known to be involved in a number of human pathologies including atherosclerosis. the purpose of this cross-sectional study was to examine the association between pre-hypertension status and oxidative stress markers [total antioxidant capacity (tac) and ma...

A high performance liquid chromatographic method for determination of rifabutin in human plasma was developed. The method involved deproteinisation of the sample with acetonitrile and analysis of the supernatant using a reversed-phase C18 column (250mm) and UV detection at a wavelength of 265nm. The assay was specifi c for rifabutin and linear from 0.025 to 10.0μg/ml. The relative standard devi...

BACKGROUND The Precision Xtra(®) meter is a promising low cost electrochemical point-of-care unit for measuring blood glucose concentration ([gluc]) in cattle blood. The meter uses an algorithm that assumes the intra-erythrocyte [gluc] equals the plasma [gluc] on a molal basis, and that the hematocrit is similar in humans and cattle. OBJECTIVES The primary objective was to determine the accur...