168 Abstract Background: Cytochemical studies on peroxidase have contributed significantly to understanding of the movement of lysosomal enzymes and secretory protein through the various compartments of secretary pathway. The peroxidase in leukocytes (myeloperoxidase) could help in determining the severity of inflammation. Aim: To determine the severity of inflammation in gingival tissue associ...

Glutathione peroxidase (glutathione--H2O2 oxidoreductase; EC 1.11.1.9) was purified to homogeneity from human placenta by using (NH4)2SO4 precipitation, ion-exchange chromatography, Sephadex gel filtration and preparative polyacrylamide-disc-gel electrophoresis. Glutathione peroxidase from human placenta is a tetramer, having 4g-atoms of selenium/mol of protein. The molecular weight of the enzy...

The method of tyramine amplification is based on the ability of HRP to catalyze the deposition of large amounts of biotinylated tyramine in the vicinity of the antigen-antibody reaction (i.e. the cellular site which contains the peroxidase detection complex). The method is based on earlier biochemical observations on the action of peroxidase upon tyramine and other related compounds (GROSS AJ a...

Hydroquinone is a nephrocarcinogen in rats but generally tests negative in standard mutagenicity assays. However, 2,3,5-tris-(glutathion-S-yl)hydroquinone, a potent nephrotoxic metabolite of hydroquinone, and 2-bromo-bis-(glutathion-S-yl)hydroquinone, another cytotoxic quinol-glutathione (GSH) conjugate, cause extensive single strand breaks in DNA in a manner that is dependent on the formation ...

Lignocelluloses are imperative structural components of plant cell wall and are profusely found in agricultural crop residues. The structural heterogeneity and recalcitrance of lignin limit the accessibility of cell wall carbohydrates for constructive exploitation. During the past decades, diverse lignin degrading enzymes were characterized to facilitate the utilization of lignocellulosic bioma...

The results of testing a new enzyme, anionic tobacco peroxidase (TOP), in various amperometric biosensors are summarized. The biochemical and electrochemical properties of the enzyme are briefly characterized. As compared to the commonly used cationic peroxidase from horseradish roots, TOP exhibits a wider optimum stability pH range, higher stability to inactivation with hydrogen peroxide, and ...

background: blindness due to cataract is a major and important problem in pakistan. the problem is expected to increase in future due to increase in tobacco smoking. the aim of study was to look for the mechanism associated with metal deposition and its effect on antioxidant enzymes in lens of smokers in karachi, pakistan. methods: 20 cataract patients were randomly selected from out patient de...

abstract background: hereditary red cell enzyme disorders are a group of non-immune/spherocytic hemolytic anemia, although these disorders are rare and they have not public health problems, the detection of these defects could help to physician in treatment and differential diagnosis. this study evaluated 5 enzymopathies in patients with hereditary non –immune/spherocytic hemolytic anemia (hnsh...