A novel C(35) terpene and its monounsaturated analogue were isolated from cultures of Acetobacter xylinum, together with traces of their C(36) homologues. These substances were found to be hopane derivatives substituted by a five-carbon chain bearing four vicinal hydroxyl groups. For the parent hydrocarbon the term bacteriohopane is proposed. The elucidation of the structures utilized high-reso...

Method for production and regeneration of Acetobacter pasterianus protoplasts is described. The protoplasts were obtained by treatment of cells with lysozyme in protoplast buffer at pH 8.0 with different osmotic stabilizers. The protoplasts were regenerated on Acetobacter medium with various osmotic stabilizers. Maximum protoplast formation was obtained in protoplast buffer with NaCl (0.5M) as ...

Bacterial cellulose is a strong and ultrapure form of cellulose produced naturally by several species of the Acetobacteraceae Its high strength, purity, and biocompatibility make it of great interest to materials science; however, precise control of its biosynthesis has remained a challenge for biotechnology. Here we isolate a strain of Komagataeibacter rhaeticus (K. rhaeticus iGEM) that can pr...

Underkofler et al. (1943) first demonstrated that pantothenic acid is an essential requirement for Acetobacter suboxydans, ATCC 621, and that this organism responds to pantolactone in a pantothenate deficient medium. j5-Alanine, however, is not able to replace pantothenic acid. Subsequently, Sarett and Cheldelin (1945) described the assay of pantolactone using the same strain of A. suboxydans. ...

A pellicle, a gel film of microbial cellulose, is a supermolecular system containing 99% of water by weight, which is closely related to an amorphous structure in it. Using ultra-small-angle neutron scattering, in order to cover over a wide range of length scales from nm to 10 microm, we examined the hierarchical amorphous structure in the microbial cellulose, which is synthesized by a bacteriu...

Cyclic diguanylic acid acts as a regulator for cellulose synthase activity in the bacterium Acetobacter xylinum. We report the x-ray crystal structure of the regulator at atomic resolution. The structure contains two independent molecules that adopt almost identical conformations. The two molecules form self-intercalated units that are stacked on each other. Two different G.G base-pairing modes...

Calcofluor White ST, stilbene derivative used commerically as an optical brightener for cellulose, increased the rate of glucose polymerization into cellulose by resting cells of the gram-negative bacterium Acetobacter xylinum. This bacterium normally produces a ribbon of cellulose that is a composite of crystalline microfibrils. In concentrations above 0.1 mM, Calcofluor disrupts the assembly ...

A total of 55 isolates of Acetobacter diazotrophicus recovered from diverse sucrose-rich host plants and from mealybugs associated with sugarcane plants were characterized by the electrophoretic mobilities of 12 metabolic enzymes. We identified seven different electrophoretic types (ETs), six of which are closely related within a genetic distance of 0.195 and exhibit high DNA-DNA homology. The ...

(72) Inventors: • PIAZZA, Pier Vincenzo F-33077 Bordeaux (FR) • VALLEE, Monique F-33077 Bordeaux (FR) • MARSICANO, Giovanni F-33077 Bordeaux (FR) • FELPIN, François-Xavier F-44322 Nantes (FR) • BELLOCCHIO, Luigi F-33077 Bordeaux (FR) • COTA, Daniela F-33077 Bordeaux (FR) • REVEST, Jean-Michel F-33077 Bordeaux (FR) • VITIELLO, Sergio F-33000 Bordeaux (FR) • SPAMPINATO, Umberto F-33077 Bordeaux (...