Objective: To run a comparison between specificity, sensitivity, PPV (positive predictive value) (PPV) & NPV (negative of the three varied H. pylori detection methods used in study. Materials and Methods: It is retrospective descriptive study that was carried out at Histopathology wing Pathology Department, Sahiwal Medical College, Sahiwal. comprised entire cases gastritis which were histop...

AIM To prepare a monoclonal antibody (EB9) against the trophozoite form of Pneumocystis carinii and to test its efficacy for detecting infection with this organism. METHOD The sensitivity and specificity of the EB9 antibody were assessed by comparing it with other conventional stains (Papanicolaou, Giemsa and Grocott) and 3F6 antibody in 33 bronchoalveolar lavage specimens from HIV positive p...

Sixty two patients diagnosed as having adult chlamydial ophthalmia were treated with oral doxycycline and roxithromycin in association and tetracycline eye wash for 2 weeks. Chlamydial ophthalmia was diagnosed by laboratory detection of the micro-organism in ocular specimens using direct immunofluorescent monoclonal antibody staining for Chlamydia trachomatis, chlamydial culture in cycloheximid...

The aim was to examine the morphology of spermatozoa with different staining methods and aimed to find the better staining methods for morphology of spermatozoa in our study. Randomized 67 patients taken for the study who were admitted to Assisted Reproductive Techniques Unit. In the first part of the study, smears were stained with Hematoxylin Eosin (HE), Toluidin Blue (TB), Giemsa, Wright, fe...

To establish a method for processing lymphoid organs suited to morphological, immunohistochemical and enzyme histochemical analyses for assessment of immunotoxicity, we examined a combination of fixation with periodate-lysine-paraformaldehyde (PLP) fixative and embedding in paraffin by the AMeX method (PLP-AMeX method). Spleen and thymus removed from monkeys and rats were fixed in PLP fixative ...

Chromatin organization in the holocentric chromosomes of the aphid Brevicoryne brassicae has been investigated at a cytological level after C-banding, NOR, Giemsa, DAPI and CMA3 staining. C-banding technique showed the presence of heterochromatic bands on both telomeres of the two X chromosomes, whereas only the longest pair of autosomes show distinct intercalary C-positive bands. Moreover, sil...

OBJECTIVES To investigate the relationship between the Helicobacter pylori (H. pylori) colonization and the grade of gastritis in the antrum and in the body of patients with duodenal ulcer (DU) or benign gastric ulcer (BGU). METHODS This study was performed in H. pylori-positive 220 DU patients and 180 BGU patients. H. pylori density was evaluated by modified Giemsa staining and CLO test, and...

OBJECTİVE: In this study, we aimed to examine of antileishmanial effect curcumin-mediated sonodynamic (SDT), photodynamic (PDT), and sonophotodynamic (SPDT) therapies with a potential mechanism against the Leishmania tropica (L.tropica) promastigotes. SPDT is new treatment modality for Leishmaniasis that combines therapies. Curcumin natural antiinflammatory agent has been used treating medical ...

Background: Bovine theileriosis is a tick-born disease in tropical and subtropical regions of the world. The study was aimed to detect Theileria sp. at genus level blood samples both local imported feedlot bulls by direct Giemsa staining, ELISA, nested polymerase chain reaction. Methods: A total 250 (125 125 bulls) were collected randomly. from five different districts Duhok Governorate Kurdist...