objective: the objective of this study is to develop and assess targeted pamam-peg nanocarrier with anti-tag72 nanobody for t-bid gene coding construct delivery into the human colonic adenocarcinoma cells.
materials and methods: nanobody (nb) coding sequence was subcloned into psj expression vector for large-scale production and then nb was purified by ni++ affinity chromatography. sds-page an...
