INTRODUCTION A 3D-nanofiber scaffold acts in a similar way to the extracellular matrix (ECM)/basement membrane that enhances the proliferation and self-renewal of stem cells. The goal of the present study was to investigate the effects of a poly L-lactic acid (PLLA) nanofiber scaffold on frozen-thawed neonate mouse spermatogonial stem cells (SSCs) and testis tissues. METHODS The isolated sper...

The study aimed to compare the effects of different cryopreservation temperatures on mechanical properties and determine optimal temperature for bones in Japanese quail. Bone biomechanical tests are getting more attention but, fresh not always available testing have a limited lifespan. Cryopreservation biological specimens is often needed during tissue preparation testing. In study, tibiotarsi ...

To evaluate the quality and safety of fish seafood meat, along with generally accepted methods, new histological research methods are used, which allow establishing microscopic changes in fresh, spoiled, canned foods. The purpose this study is to examine structure salted meat (Herring, Pollock) (squid, mussels) by freezing. It was confirmed that composed skeletal muscle, fibrous connective (end...