Basic fibroblast growth factor (bFGF) regulates skin wound healing; however, the underlying mechanism remains to be defined. In the present study, we determined the effects of bFGF on the regulation of cell growth as well as collagen and fibronectin expression in fibroblasts from normal human skin and from hypertrophic scars. We then explored the involvement of mitochondria in mediating bFGF-in...

Fibroblas berperan dalam proses penyembuhan luka dengan pembentukan pembuluh darah, penggerakan serta proliferasi, deposit matriks ekstraseluler dan remodeling jaringan. Pada luka, netrofil makrofag akan mengalami peningkatan penggunaan oksigen sehingga memproduksi ROS, produk ini menyebabkan stres oksidatif pada fibroblas yang mempengaruhi migrasi proliferasi selama luka. Kandungan gugus amino...

Objective — to assess the efficacy and safety of intradermal injections allogeneic fibroblasts into non-healing wounds in a patient with junctional epidermolysis bullosa. Material Methods A 49-year-old intermediate bullosa was injected intradermally base 1 mL suspension fibroblasts, which contained 5×106 cells/mL, 10×106 20×106 cells/mL. Immunofluorescence mapping exhibited reduced β3 chain lam...

Proteomic profiling of Sengers patient fibroblasts and AGK knockout models identifies remodeling the mitochondrial proteome, including one-carbon metabolism enzymes, inner membrane serine transporters, sideroflexins, Complex I subunits assembly factors.

Fibroblasts are at the heart of cardiac function and are the principal determinants of cardiac fibrosis. Nevertheless, cardiac fibroblasts remain poorly characterized in molecular terms. Evidence is evolving that the cardiac fibroblast is a highly heterogenic cell population, and that such heterogeneity is caused by the distinct origins of fibroblasts in the heart. Cardiac fibroblasts can deriv...

AIMS Crosstalk between cardiomyocytes and fibroblasts in physiological conditions and during disease remains poorly defined. Previous studies have shown that fibroblasts and myocytes interact via paracrine communication, but several experimental confounding factors, including the use of immature myocytes and the induction of alpha-smooth muscle actin (α-SMA) expression in fibroblasts by prolong...

BACKGROUND Myofibroblasts play a crucial role in tissue repair. The functional similarities and differences between myofibroblasts and fibroblasts are not fully understood because they have not been separately isolated from a living body. The purpose of this study was to establish a method for the direct isolation of myofibroblasts and fibroblasts from injured lungs by using fluorescence-activa...

Matrix metalloproteinase-9 (MMP-9) is a matrix-degrading enzyme implicated in many biological processes, including inflammation. It is produced by many cells, including fibroblasts. When cultured in three-dimensional (3D) collagen gels, fibroblasts contract the surrounding matrix, a function that is thought to model the contraction that characterizes both normal wound repair and fibrosis. The c...

This study investigated the ability to reprogramming human fibroblast cells into hepatocyte-like by overexpressing HNF4a gene. The isolated and primary cultured from foreskin donated healthy patients. gene overexpressed through Doxycilin activatedthe vectors Tet-on-eGFP-HNF4a. after process were evaluated observing morphological changes, immunofluorescence staining assess expression of proteins...