Background: The DNA-dependent protein kinase (DNA-PK) plays a critical role in the non-homologous end joining (NHEJ) double-strand break (DSB) repair pathway and DNA damage response (DDR). Consequently, blocking DNA-PK activity is being pursued as therapeutic strategy for treatment of cancer combination with ionizing radiation (IR). Towards developing new class inhibitors, our laboratory previo...

Dpb11 is required for the initiation of DNA replication in budding yeast. We found that Dpb11 binds tightly to single-stranded DNA (ssDNA) or branched DNA structures, while its human homolog, TopBP1, binds tightly to branched-DNA structures. We also found that Dpb11 binds stably to CDK-phosphorylated RPA, the eukaryotic ssDNA binding protein, in the presence of branched DNA. A Dpb11 mutant spec...

The silver-containing porphyrin Ag-meso-tetra(4-N-hydroxyethylpyridyl)porphyrin (AgTOEPyP4) was investigated as a potential anticancer drug using spectrophotometric, calorimetric and electrophoretic measurements. studies were conducted on healthy, cancer-induced, treated by AgTOEPyP4 white mice. DNA isolated from the liver tumor tissues of Melting curves studied in presence various stoichiometr...

Quantifying DNA-protein interaction using DNA microarrays are gaining increasing attention due to their ability to profile specificity of interactions in a high-throughput manner. This paper describes a new approach that used the ability of ssDNA-dsDNA probe to complex with DNA binding proteins in the solution phase and then spatially immobilized onto microarray through specific DNA hybridizati...

The chemical structure of oligonucleotide analogues dictates the conformation analogue oligomers, their ability to hybridize complementary DNA and RNA, stability degradation pharmacokinetic properties. In a study aimed at investigating new featuring neutral backbone, we explored oligomers containing morpholino-peptide backbone bind oligonucleotides. Circular Dichroism studies revealed our inter...

Electrically controllable deoxyribonuclic acid (DNA) nanolevers are used to investigate the binding interaction between Influenza A/Aichi/2/1968 and peptide called “PeB”, which specifically binds viral surface protein hemagglutinin. PeB is immobilized on gold electrodes of a “switchSENSE” biochip by conjugation DNA-strands that hybridized complementary anchors. The surface-tethered DNA strand c...

the aggregation behavior of anionic cobalt(ii) 4,4′,4ʺ,4‴-tetrasulfonated phthalocyanine, [co(tspc)4-] was studied at its various concentrations and different ionic strengths using optical absorption and resonance light scattering (rls) spectroscopies in 5 mm phosphate buffer, ph 7.0 at 25 °c. the results show no aggregation behavior at concentration range of 5.1 × 10-6-7.5 × 10-5 m. also, the ...