In vitro germplasms of ipecac (Cephaelis ipecacuanha Rich.), an important medicinal plant, maintained through reduced growth conditions for more than 12-years, were used as source material for micropropagation. MS with different combinations of Kn (2 mg/l), BAP (2 mg/l), 2iP (2-3 mg/l), NAA (0.2 mg/l) and adenine (40-100 mg/l) as additive was used to induce fresh multiplication of shoots from t...

Tissue culture is considered as an alternative tool for garlic breeding as traditional breeding is not possible due to its sterility. A study was carried out to develop a protocol of regeneration in Bangladesh local garlic. Shoot and root meristems of garlic cv. Bangladesh local were cultured on Murashige and Skoog (MS) medium containing various growth regulator combinations. Regeneration from ...

In vitro regeneration of shoots from leaf explants of the Paradise tree (Melia azedarach L.) was studied. Three different portions (proximal portion, distal portion and rachis of the leaflets) of three developmental stages (folded, young still expanding and completely expanded) of leaves of 10-15 year old plants of seven genotypes were cultured on Murashige and Skoog (1962) medium (MS) suppleme...

of Dissertation Presented to the Graduate School of the University of Florida in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy INDIRECT SHOOT ORGANOGENESIS AND SELECTION OF SOMACLONAL VARIATION IN Dieffenbachia 11 By Xiuli Shen May 2007 Chair: Michael E Kane Cochair: Jianjun Chen Major: Horticultural Science A series of experiments were conducted to investigate ...

two separate factorial experiments were conducted to study the effects of explant sources, plant growth regulators, sucrose concentrations, and light conditions on in vitro cormlet formation of freesia (freesia ×hybrida bailey 'argenta'). interestingly, it was observed that the pupae had lower contamination levels compared to mother corms. using 40% sodium hypochlorite solution for 40 min, cont...

two separate factorial experiments were conducted to study the effects of explant sources, plant growth regulators, sucrose concentrations, and light conditions on in vitro cormlet formation of freesia (freesia ×hybrida bailey 'argenta'). interestingly, it was observed that the pupae had lower contamination levels compared to mother corms. using 40% sodium hypochlorite solution for 40...

A simple micropropagation method from shoot tip explants was reported here for Clitoria ternatea L., an economically important medicinal plant. High frequency of micro shoots were obtained from this explants on MS medium supplemented with various concentrations of Indole Acetic acid (IAA) (0.5 2.0 mg/l) and KN (0.5 -2.0 mg/l). Maximum number of shoots was obtained from shoot tip explants in the...

Ridge gourd (Luffa acutangula L. Roxb) is a popular and nutritious vegetable in Bangladesh. The present investigation was undertaken to establish an efficient in vitro regeneration system of ridge gourd from immature embryo and cotyledon explants. Immature embryos and cotyledons were collected from immature seeds and culture on MS medium supplemented with various concentration and combination o...

Vitex trifolia is a shrub species with popular use as a medicinal plant, for which leaves, roots and flowers have been reported to heal different distresses. The increasing exploitation of these plants has endangered its conservation, and has importantly justified the use of biotechnological tools for their propagation. Our aim was to present an efficient protocol for plant regeneration through...