PURPOSE Tumor-specific replication-selective oncolytic virotherapy is a promising antitumor therapy for induction of cell death in tumor cells but not of normal cells. We previously developed an oncolytic adenovirus, OBP-301, that kills human epithelial malignant cells in a telomerase-dependent manner. Recent evidence suggests that nonepithelial malignant cells, which have low telomerase activi...

In this study, our previously reported novel synthetic gene encoding 166 residues of interferon-? was used for an efficient expression of IFN-?. The synthetic gene was cloned into pET21a expression vector and transferred into E. coli. Recombinant protein was over-expressed in the E. coli. Identity of the recombinant protein was confirmed by western blot analysis. The recombinant protein was bio...

Ribavirin (1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamide), a new synthetic nucleoside, inhibited murine cytomegalovirus in cell culture. This was shown by the inhibition of viral cytopathic effect and plaque formation, as well as reduction in the yield of virus. Despite this in vitro antiviral effect, ribavirin did not protect mice from mortality produced by a high inoculum of cytomegalov...

CD4+ T lymphocytes of individuals infected with human immunodeficiency virus type 1 (HIV-1) exhibit a qualitative defect in their ability to mount memory responses to previously encountered antigens although their responses to mitogens remain normal. T cells responsible for memory responses can be distinguished from naive T cells based on differential expression of isoforms of the tyrosine phos...

The complete genome sequences of a cytopathic/noncytopathic pair of border disease viruses (BDV) that were isolated from a sheep with mucosal disease-like lesions were determined. The cytopathic virus possessed an insert of 288 nucleotides that was derived from the Jiv cellular transcript and had 37 nucleotide differences from the noncytopathic virus.

Through bioactivity-guided fractionation, eight phorbol diesters, including five new ones (1-5), were isolated from the seeds of Croton tiglium collected in Egypt. 12-O-Acetylphorbol-13-decanoate (6) and 12-O-decanoylphorbol-13-(2-methylbutyrate) (4) potently inhibited the HIV-1-induced cytopathic effect on MT-4 cells (IC100 values of 7.6 ng/ml and 7.81 micrograms/ml, and CC0 values of 62.5 mic...

A 23-year-old woman gave a history of intermittent post-coital vaginal spotting, and a Papanicolaou smear indicated the presence of a low-grade squamous intraepithelial lesion and cellular changes due to the cytopathic effect of human papillomavirus (HPV) infection. Colposcopic examination showed a circumferential exophytic lesion (Fig 1). Multiple biopsies were performed; histological examinat...