Cortisone acetate test was performed in twelve young adult patients with diabetes mellitus type 1, after dexamethasone administration to suppress endogenous cortisol production. Previous screening revealed that all of the subjects had peak cortisol responses in the range from subnormal to normal, as determined by a low-dose Synacthen test. The aim was to find out whether these patients would ex...

Previous experiments have shown that the growth of ceils explanted from tuberculin-sensitive animals may be inhibited if tuberculin is added to the culture menstrnum (1). Tissue culture techniques also have been employed in studies of the mechanism of action of cortisone. The direct effect of this compound upon such diverse physiologic phenomena as heart muscle contraction (2) and renal tubular...

Steroid hormone concentrations are mostly determined by using different body fluids as matrices and applying immunoassay techniques. However, usability of these approaches may be restricted for several reasons, including ethical barriers to invasive sampling. Therefore, we developed an ultra-performance LC-MS-MS method for high-throughput determination of concentrations of cortisol, cortisone, ...

1. Marked hypertension was induced in adrenalectomized rats on a sodium-restricted intake by the administration of cortisone acetate; on a similar regimen, adrenalectomized rats receiving DCA failed to become hypertensive. 2. The incorporation of a liberal amount of sodium in the diet of adrenalectomized rats receiving cortisone acetate resulted in a lesser degree of hypertension; whereas on a ...

The eosinopenic response to stress has been recognized since Zappert (1893) described its occurrence m certain infections, and in recent years it has been included among the phenomena of the alarm reaction (Selye, 1949). It was also shown that a decrease in the number of circulating eosinophils followed the injection of corticotrophin or cortisone, and Hills, Forsham and Finch (1948) concluded ...

THERE are few reports in the literature of experimental work dealing with the effect of cortisone upon corneal vascularization, and the evidence so fair advanced is partly conflicting (Duke-Elder and Ashton, 1951), but there is already some indication from clinical observers that cortisone exerts a considerable inhibitory influence on the formation of ne\v vessels in the diseased cornea. The ex...

The nuclcar histones of control and antigen-affected lymphocytcs and thymocytes were measured in terms of their intensity of staining with ammoniacal-silver (A-S) and alkaline fast green (FG). Subcutaneous injection of full strength and doubly concentrated tetanus toxoid into the upper forelimb was followed by an acute decrease in the intensity of thc FG and A-S staining of the thymocyte nuclea...

The nuclcar histones of control and antigen-affected lymphocytcs and thymocytes were measured in terms of their intensity of staining with ammoniacal-silver (A-S) and alkaline fast green (FG). Subcutaneous injection of full strength and doubly concentrated tetanus toxoid into the upper forelimb was followed by an acute decrease in the intensity of thc FG and A-S staining of the thymocyte nuclea...

The chromatographic behavior of cortisol and cortisone using a micellar medium of sodium dodecyl sulphate (SDS) as surfactant, a Hypersil C18 (150- x 3.2-mm i.d., 5 microm) column, a flow rate of 0.5 mL/min, and UV absorbance detection at 245 nm is described. The effect of several organic modifiers and the surfactant concentration on the separation is studied. A mobile phase of 18 mM SDS and 8....

The chemical syntheses of the 21-dehydro derivatives’ of a number of C1r a-ketolsteroids, including cortisone (3),2 cortisol (3), deoxycorticosterone (4), and Al-cortisone (5), have been described. The biological activities of the 11-oxygenated 21dehydrocorticosteroids were found to be similar to the corresponding 2l-hydroxysteroids. Schneider (6) reported that aqueous extracts of rat liver ace...